2026-06-14T03:36:01Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/165122024-10-31T11:51:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Coronel Lopez, Raquel
author
Bernabeu-Zornoza, Adela
author
Palmer, Charlotte
author
González-Sastre, Rosa
author
Rosca, Andreea
author
Mateos-Martínez, Patricia
author
López-Alonso, Victoria
author
Liste-Noya, Isabel
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
University of Alcalá (España)
2023-09-26T12:46:26Z
2023-09-26T12:46:26Z
2023-08-19
Int J Mol Sci. 2023 Aug 19;24(16):12964.
10.3390/ijms241612964
1422-0067
International journal of molecular sciences
37629148
http://hdl.handle.net/20.500.12105/16512
Numerous studies have focused on the pathophysiological role of amyloid precursor protein (APP) because the proteolytic processing of APP to β-amyloid (Aβ) peptide is a central event in Alzheimer's disease (AD). However, many authors consider that alterations in the physiological functions of APP are likely to play a key role in AD. Previous studies in our laboratory revealed that APP plays an important role in the differentiation of human neural stem cells (hNSCs), favoring glial differentiation (gliogenesis) and preventing their differentiation toward a neuronal phenotype (neurogenesis). In the present study, we have evaluated the effects of APP overexpression in hNSCs at a global gene level by a transcriptomic analysis using the massive RNA sequencing (RNA-seq) technology. Specifically, we have focused on differentially expressed genes that are related to neuronal and glial differentiation processes, as well as on groups of differentially expressed genes associated with different signaling pathways, in order to find a possible interaction between them and APP. Our data indicate a differential expression in genes related to Notch, Wnt, PI3K-AKT, and JAK-STAT signaling, among others. Knowledge of APP biological functions, as well as the possible signaling pathways that could be related to this protein, are essential to advance our understanding of AD.
eng
Amyloid precursor protein
Neural stem cells
Neurogenesis
Gliogenesis
Signaling pathways
RNA sequencing
Amyloid Precursor Protein (APP) Regulates Gliogenesis and Neurogenesis of Human Neural Stem Cells by Several Signaling Pathways
research article
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