2026-06-14T03:40:37Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/165122024-10-31T11:51:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc Coronel Lopez, Raquel author Bernabeu-Zornoza, Adela author Palmer, Charlotte author González-Sastre, Rosa author Rosca, Andreea author Mateos-Martínez, Patricia author López-Alonso, Victoria author Liste-Noya, Isabel author 2023-08-19 Numerous studies have focused on the pathophysiological role of amyloid precursor protein (APP) because the proteolytic processing of APP to β-amyloid (Aβ) peptide is a central event in Alzheimer's disease (AD). However, many authors consider that alterations in the physiological functions of APP are likely to play a key role in AD. Previous studies in our laboratory revealed that APP plays an important role in the differentiation of human neural stem cells (hNSCs), favoring glial differentiation (gliogenesis) and preventing their differentiation toward a neuronal phenotype (neurogenesis). In the present study, we have evaluated the effects of APP overexpression in hNSCs at a global gene level by a transcriptomic analysis using the massive RNA sequencing (RNA-seq) technology. Specifically, we have focused on differentially expressed genes that are related to neuronal and glial differentiation processes, as well as on groups of differentially expressed genes associated with different signaling pathways, in order to find a possible interaction between them and APP. Our data indicate a differential expression in genes related to Notch, Wnt, PI3K-AKT, and JAK-STAT signaling, among others. Knowledge of APP biological functions, as well as the possible signaling pathways that could be related to this protein, are essential to advance our understanding of AD. Int J Mol Sci. 2023 Aug 19;24(16):12964. 10.3390/ijms241612964 1422-0067 International journal of molecular sciences 37629148 http://hdl.handle.net/20.500.12105/16512 Amyloid precursor protein Neural stem cells Neurogenesis Gliogenesis Signaling pathways RNA sequencing Amyloid Precursor Protein (APP) Regulates Gliogenesis and Neurogenesis of Human Neural Stem Cells by Several Signaling Pathways