2026-04-29T06:16:10Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/164662024-09-27T09:14:06Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Campos-Iglesias, Diana author Fraile, Julia M author Bretones, Gabriel author Montero, Alejandro A author Bonzon-Kulichenko, Elena author Vazquez, Jesus author López-Otín, Carlos author Freije, José M P author 2023-01-26 The spindle assembly checkpoint (SAC) is an essential mechanism that ensures the accurate chromosome segregation during mitosis, thus preventing genomic instability. Deubiquitinases have emerged as key regulators of the SAC, mainly by determining the fate of proteins during cell cycle progression. Here, we identify USP49 deubiquitinase as a novel regulator of the spindle checkpoint. We show that loss of USP49 in different cancer cell lines impairs proliferation and increases aneuploidy. In addition, USP49-depleted cells overcome the arrest induced by the SAC in the presence of nocodazole. Finally, we report new binding partners of USP49, including ribophorin 1, USP44, and different centrins. Cell Death Dis. 2023 Jan 26;14(1):60. http://hdl.handle.net/20.500.12105/16466 36702832 10.1038/s41419-023-05600-x 2041-4889 Cell death & disease USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy.