2026-05-17T11:53:53Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/164632024-09-27T09:59:45Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Sendra, Miquel
author
de Dios Hourcade, Juan
author
Temiño, Susana
author
Sarabia, Antonio J
author
Ocaña, Oscar H
author
Domínguez, Jorge N
author
Torres, Miguel
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación La Caixa
funder
Unión Europea. Comisión Europea. H2020
funder
Centro Nacional de Investigaciones Cardiovasculares Carlos III (España)
2023-09-15T08:44:01Z
2023-09-15T08:44:01Z
2023-02-15
Development. 2023 Feb 15;150(3):dev201206.
10.1242/dev.201206
1477-9129
Development (Cambridge, England)
36734327
http://hdl.handle.net/20.500.12105/16463
Tracing and manipulating cells in embryos are essential to understand development. Lipophilic dye microinjections, viral transfection and iontophoresis have been key to map the origin of the progenitor cells that form the different organs in the post-implantation mouse embryo. These techniques require advanced manipulation skills and only iontophoresis, a demanding approach of limited efficiency, has been used for single-cell labelling. Here, we perform lineage tracing and local gene ablation using cell-permeant Cre recombinase (TAT-Cre) microinjection. First, we map the fate of undifferentiated progenitors to the different heart chambers. Then, we achieve single-cell recombination by titrating the dose of TAT-Cre, which allows clonal analysis of nascent mesoderm progenitors. Finally, injecting TAT-Cre to Mycnflox/flox embryos in the primitive heart tube revealed that Mycn plays a cell-autonomous role in maintaining cardiomyocyte proliferation. This tool will help researchers identify the cell progenitors and gene networks involved in organ development, helping to understand the origin of congenital defects.
eng
Cre recombinase microinjection for single-cell tracing and localised gene targeting.
journal article
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URL
https://repisalud.isciii.es/bitstreams/de54a09f-a219-427e-bbe6-1fe416679142/download
File
MD5
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application/pdf
Cre recombinase microinjection_Development_2023.pdf
URL
https://repisalud.isciii.es/bitstreams/1c1a8eb2-9695-4fd7-bc67-8d377e25803d/download
File
MD5
47670f007fcf0547086af94298aa5011
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text/plain
Cre recombinase microinjection_Development_2023.pdf.txt