2026-08-12T20:37:27Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/164622024-05-27T19:32:34Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Cortenbach, Kimberley R G
author
Morales Cano, Daniel
author
Meek, Jelena
author
Gorris, Mark A J
author
Staal, Alexander H J
author
Srinivas, Mangala
author
Jolanda M de Vries, I
author
Fog Bentzon, Jacob
author
van Kimmenade, Roland R J
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Fundación ProCNIC
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2023-09-15T08:33:17Z
2023-09-15T08:33:17Z
2023-02
Int J Cardiol Heart Vasc. 2022 Aug 24;44:101111.
10.1016/j.ijcha.2022.101111
2352-9067
International journal of cardiology. Heart & vasculature
36820389
http://hdl.handle.net/20.500.12105/16462
BACKGROUND
Aim of this study was to investigate immune cells and subsets in different stages of human coronary artery disease with a novel multiplex immunohistochemistry (mIHC) technique.
METHODS
Human left anterior descending coronary artery specimens were analyzed: eccentric intimal thickening (N = 11), pathological intimal thickening (N = 10), fibroatheroma (N = 9), and fibrous plaque (N = 9). Eccentric intimal thickening was considered normal, and pathological intimal thickening, fibroatheroma, and fibrous plaque were considered diseased coronary arteries. Two mIHC panels, consisting of six and five primary antibodies, autofluoresence, and DAPI, were used to detect adaptive and innate immune cells. Via semi-automated analysis, (sub)types of immune cells in whole plaques and specific plaque regions were quantified.
RESULTS
Increased numbers of CD3+ T cells (P < 0.001), CD20+ B cells (P = 0.013), CD68+ macrophages (P = 0.003), CD15+ neutrophils (P = 0.017), and CD31+ endothelial cells (P = 0.024) were identified in intimas of diseased coronary arteries compared to normal. Subset analyses of T cells and macrophages showed that diseased coronary arteries contained an abundance of CD3+CD8- non-cytotoxic T cells and CD68+CD206- non-M2-like macrophages. Proportions of CD3+CD45RO+ memory T cells were similar to normal coronary arteries. Among pathological intimal thickening, fibroatheroma, and fibrous plaque, all immune cell numbers and subsets were similar.
CONCLUSIONS
The type of immune response does not differ substantially between different stages of plaque development and may provide context for mechanistic research into immune cell function in atherosclerosis. We provide the first comprehensive map of immune cell subtypes across plaque types in coronary arteries demonstrating the potential of mIHC for vascular research.
eng
Topography of immune cell infiltration in different stages of coronary atherosclerosis revealed by multiplex immunohistochemistry.
journal article
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Topography of immune cell_Int J Cardiol Heart Vasc_2022.pdf.txt