2026-04-18T15:59:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/164452024-09-27T08:30:44Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Marina-Zarate, Ester
author
Rodríguez-Ronchel, Ana
author
Gomez, Manuel J
author
Sanchez-Cabo, Fatima
author
Ramiro, Almudena R
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación La Caixa
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Fundación ProCNIC
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2023-09-12T10:07:17Z
2023-09-12T10:07:17Z
2023-03-17
iScience. 2023 Feb 2;26(3):106106.
http://hdl.handle.net/20.500.12105/16445
36852270
10.1016/j.isci.2023.106106
2589-0042
iScience
CTCF is a DNA-binding protein which plays critical roles in chromatin structure organization and transcriptional regulation; however, little is known about the functional determinants of different CTCF-binding sites (CBS). Using a conditional mouse model, we have identified one set of CBSs that are lost upon CTCF depletion (lost CBSs) and another set that persists (retained CBSs). Retained CBSs are more similar to the consensus CTCF-binding sequence and usually span tandem CTCF peaks. Lost CBSs are enriched at enhancers and promoters and associate with active chromatin marks and higher transcriptional activity. In contrast, retained CBSs are enriched at TAD and loop boundaries. Integration of ChIP-seq and RNA-seq data has revealed that retained CBSs are located at the boundaries between distinct chromatin states, acting as chromatin barriers. Our results provide evidence that transient, lost CBSs are involved in transcriptional regulation, whereas retained CBSs are critical for establishing higher-order chromatin architecture.
eng
Low-affinity CTCF binding drives transcriptional regulation whereas high-affinity binding encompasses architectural functions.
journal article
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URL
https://repisalud.isciii.es/bitstreams/c650c911-7ed2-40d6-9616-4f5c089d6a1d/download
File
MD5
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application/pdf
Low_affinity CTCF_iScience_2023.pdf
URL
https://repisalud.isciii.es/bitstreams/3bef78d8-1644-4b61-b8ad-a4a25fb69332/download
File
MD5
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text/plain
Low_affinity CTCF_iScience_2023.pdf.txt