2026-05-16T22:10:09Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/164282025-05-08T15:14:23Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Corrêa dos Santos, Renato Augusto
author
Mead, Matthew E
author
Steenwyk, Jacob L
author
Rivero-Menendez, Olga
author
Alastruey-Izquierdo, Ana
author
Goldman, Gustavo Henrique
author
Rokas, Antonis
funder
São Paulo Research Foundation
funder
Howard Hughes Medical Institute
funder
Vanderbilt University (Estados Unidos)
funder
National Science Foundation (Estados Unidos)
funder
NIH - National Institute of Allergy and Infectious Diseases (NIAID) (Estados Unidos)
funder
National Council for Scientific and Technological Development (Brasil)
2023-09-07T13:43:02Z
2023-09-07T13:43:02Z
2021-09
Front. Fungal Biol. 2021;2:723051.
10.3389/ffunb.2021.723051
2673-6128
Frontiers in Fungal Biology
http://hdl.handle.net/20.500.12105/16428
Certain Aspergillus fungi cause aspergillosis, a set of diseases that typically affect immunocompromised individuals. Most cases of aspergillosis are caused by Aspergillus fumigatus, which infects millions of people annually. Some closely related so-called cryptic species, such as Aspergillus lentulus, can also cause aspergillosis, albeit at lower frequencies, and they are also clinically relevant. Few antifungal drugs are currently available for treating aspergillosis and there is increasing worldwide concern about the presence of antifungal drug resistance in Aspergillus species. Furthermore, isolates from both A. fumigatus and other Aspergillus pathogens exhibit substantial heterogeneity in their antifungal drug resistance profiles. To gain insights into the evolution of antifungal drug resistance genes in Aspergillus, we investigated signatures of positive selection in 41 genes known to be involved in drug resistance across 42 susceptible and resistant isolates from 12 Aspergillus section Fumigati species. Using codon-based site models of sequence evolution, we identified ten genes that contain 43 sites with signatures of ancient positive selection across our set of species. None of the sites that have experienced positive selection overlap with sites previously reported to be involved in drug resistance. These results identify sites that likely experienced ancient positive selection in Aspergillus genes involved in resistance to antifungal drugs and suggest that historical selective pressures on these genes likely differ from any current selective pressures imposed by antifungal drugs.
eng
Section Fumigati
Antifungal drug resistance
Positive selection
Cryptic species
Azole
Aspergillus fumigatus
Echinocandin
Examining Signatures of Natural Selection in Antifungal Resistance Genes Across Aspergillus Fungi
research article
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ExaminingSignaturesNaturalSelection_2021.pdf
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ExaminingSignaturesNaturalSelection_2021.pdf.txt
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