2026-05-23T16:43:26Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/164182025-04-01T09:58:31Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Rojas-Gómez, Amelia author Dosil, Sara G author Chichón, Francisco Javier author Fernández-Gallego, Nieves author Ferrarini, Alessia author Calvo, Enrique author Calzada-Fraile, Diego author Requena, Silvia author Otón, Joaquin author Serrano, Alvaro author Tarifa, Rocio author Arroyo, Montserrat author Sorrentino, Andrea author Pereiro, Eva author Vazquez, Jesus author Valpuesta, José M author Sánchez-Madrid, Francisco author Martín-Cófreces, Noa B author 2023-06 Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganelle contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT. J Extracell Vesicles. 2023 Jun;12(6):e12333. 10.1002/jev2.12333 2001-3078 Journal of extracellular vesicles 37328936 http://hdl.handle.net/20.500.12105/16418 Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics.