2026-04-18T21:52:33Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/164022024-09-21T10:57:57Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Arderiu, Gemma
author
Mendieta, Guiomar
author
Gallinat, Alex
author
Lambert, Carmen
author
Díez-Caballero, Alberto
author
Ballesta, Carlos
author
Badimon, Lina
funder
Plan Nacional de I+D+i (España)
funder
Centro de Investigación Biomédica en Red - CIBERCV (Enfermedades Cardiovasculares)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación Jesús Serra
2023-09-04T14:25:53Z
2023-09-04T14:25:53Z
2023-01-03
Int J Mol Sci. 2023 Jan 3;24(1):827.
http://hdl.handle.net/20.500.12105/16402
36614270
10.3390/ijms24010827
1422-0067
International journal of molecular sciences
Obesity is associated with metabolic disorders such as insulin resistance and type 2 diabetes mellitus (T2DM), further increasing an already heightened cardiovascular risk. Here, amongst obese class III bariatric surgery patients, we have investigated the effect of T2DM in serum and in two, same patient, adipose tissue (AT) depots through proteomic profile expression analyses. Serum and AT samples from subcutaneous (SAT) and visceral (VAT) fat were collected during bariatric surgery. Bead-based targeted multiplex assay systems were used to simultaneously detect and quantify multiple targets in serum samples (targeted proteomics) and analyze changes in adipokine serum composition. AT samples were assessed through an untargeted proteomics approach. Through a systems biology analysis of the proteomic data, information on the affected biological pathways was acquired. In obese class III individuals, the presence of T2DM induced a significantly higher systemic release of ghrelin, GLP-1, glucagon, MMP3, BAFF, chitinase 3-like 1, TNF-R1 and TNF-R2, and a lower systemic release of IL-8. SAT and VAT proteomes belonging to the same patient showed significant differences in local protein content. While the proteins upregulated in VAT were indicative of metabolic dysregulation, SAT protein upregulation suggested adequate endocrine regulation. The presence of T2DM significantly affected VAT protein composition through the upregulation of dysregulating metabolic pathways, but SAT protein composition was not significantly modified. Our results show that T2DM induces metabolic dysregulation in obese individuals with changes in systemic marker levels and impairment of proteostasis in VAT but not in SAT.
eng
Type 2 Diabetes in Obesity: A Systems Biology Study on Serum and Adipose Tissue Proteomic Profiles.
journal article
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Type 2 Diabetes in Obesity_Int J Mol Sci_2023.pdf
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https://repisalud.isciii.es/bitstreams/debf0b79-6ae4-4cd7-b4de-c051ad197c5a/download
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Type 2 Diabetes in Obesity_Int J Mol Sci_2023.pdf.txt