2026-04-27T01:59:26Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/163542025-06-25T10:30:27Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616col_20.500.12105_25179
Repisalud
author
Fernández-Tabanera, Enrique
author
Garcia-Garcia, Laura
author
Rodriguez-Martin, Carlos
author
Cervera Mayor, Saint Thomas
author
Gonzalez-Gonzalez, Laura
author
Robledo, Cristina
author
Josa, Santiago
author
Martinez Rodríguez, Selene
author
Chapado, Luis
author
Monzon-Fernandez, Sara
author
Melero-Fernández de Mera, Raquel María
author
Alonso, Javier
funder
Instituto de Salud Carlos III
funder
Asociación Pablo Ugarte contra el cáncer infantil
funder
Candela Ribera. Asociación contra el sarcoma de Ewing
funder
Asociación Todos somos Iván
funder
Fundación la Sonrisa de Alex para la investigación y el tratamiento del sarcoma de Ewing
funder
Centro de Investigación Biomédica en Red - CIBERER (Enfermedades Raras)
funder
Asociación Infantil Oncológica de Madrid. La hucha de Tomás
2023-08-28T08:51:29Z
2023-08-28T08:51:29Z
2023-07-21
Int J Mol Sci. 2023 Jul 21;24(14):11774.
http://hdl.handle.net/20.500.12105/16354
37511533
10.3390/ijms241411774
1422-0067
International journal of molecular sciences
The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells.
eng
Ewing sarcoma
CD44
EWSR1::FLI1
Cell migration
Cell invasiveness
CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells
research article
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