2026-04-27T01:59:25Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/163542025-06-25T10:30:27Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616col_20.500.12105_25179

00925njm 22002777a 4500 dc Fernández-Tabanera, Enrique author Garcia-Garcia, Laura author Rodriguez-Martin, Carlos author Cervera Mayor, Saint Thomas author Gonzalez-Gonzalez, Laura author Robledo, Cristina author Josa, Santiago author Martinez Rodríguez, Selene author Chapado, Luis author Monzon-Fernandez, Sara author Melero-Fernández de Mera, Raquel María author Alonso, Javier author 2023-07-21 The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells. Int J Mol Sci. 2023 Jul 21;24(14):11774. http://hdl.handle.net/20.500.12105/16354 37511533 10.3390/ijms241411774 1422-0067 International journal of molecular sciences Ewing sarcoma CD44 EWSR1::FLI1 Cell migration Cell invasiveness CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells