2026-07-22T20:05:49Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/163422024-11-28T17:39:44Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961col_20.500.12105_16987
Repisalud
author
de la Torre-Tarazona, Humberto Erick
author
González-Robles, Alba
author
Cascajero Díaz, Almudena
author
Jiménez Santana, Paloma
author
Miró, José María
author
Sánchez-Palomino, Sonsoles
author
Alcamí, José
author
Buzón, Maria José
author
García-Pérez, Javier
author
Hospital Clinic HIV Investigators
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación La Marató TV3
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
funder
Fundación La Caixa
funder
Consorci Institut D Investigacions Biomediques August Pi I Sunyer
2023-08-25T08:13:50Z
2023-08-25T08:13:50Z
2023-02
J Med Virol. 2023 Feb;95(2):e28543.
10.1002/jmv.28543
1096-9071
Journal of medical virology
36727646
http://hdl.handle.net/20.500.12105/16342
The presence of neutralizing antibodies (NAbs) is a major correlate of protection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Thus, different in vitro pseudoviruses-based assays have been described to detect NAbs against SARS-CoV-2. However, the determination of NAbs against SARS-CoV-2 in people living with HIV (PLWH) through HIV-based pseudoparticles could be influenced by cross-neutralization activity or treatment, impeding accurate titration of NAbs. Two assays were compared using replication-defective HIV or VSV-based particles pseudotyped with SARS-CoV-2 spike to measure NAbs in COVID-19-recovered and COVID-19-naïve PLWH. The assay based on HIV-pseudoparticles displayed neutralization activity in all COVID-19-recovered PLWH with a median neutralizing titer 50 (NT50) of 1417.0 (interquartile range [IQR]: 450.3-3284.0), but also in 67% of COVID-19-naïve PLWH (NT50: 631.5, IQR: 16.0-1535.0). Regarding VSV-pseudoparticles system, no neutralization was observed in COVID-19-naïve PLWH as expected, whereas in comparison with HIV-pseudoparticles assay lower neutralization titers were measured in 75% COVID-19-recovered PLWH (NT50: 100.5; IQR: 20.5-1353.0). Treatment with integrase inhibitors was associated with inaccurate increase in neutralization titers when HIV-based pseudoparticles were used. IgG purification and consequent elimination of drugs from samples avoided the interference with retroviral cycle and corrected the lack of specificity observed in HIV-pseudotyped assay. This study shows methodological alternatives based on pseudoviruses systems to determine specific SARS-CoV-2 neutralization titers in PLWH.
eng
HIV‐1
Neutralization
Pseudoviruses
SARS-CoV-2
Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
research article
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TreatmentIntegraseInhibitorsSARS-CoV-2_2023.pdf
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Supplementary_TreatmentIntegraseInhibitorsSARS-CoV-2_2023.pdf
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TreatmentIntegraseInhibitorsSARS-CoV-2_2023.pdf.txt
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