2026-04-29T06:16:05Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/163382024-09-27T22:36:48Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Elmahallawy, Ehab Kotb
author
Gareh, Ahmed
author
Abu-Okail, Akram
author
Köster, Pamela Carolina
author
Dashti, Alejandro
author
Asseri, Jamal
author
Gouda, Asmaa Aboelabbas
author
Mubaraki, Murad A
author
Mohamed, Sara Abdel-Aal
author
Mohamed, Yasser M
author
Hassan, Ehssan Ahmed
author
Elgendy, Mohamed
author
Hernández-Castro, Carolina
author
Bailo-Barroso, Begoña
author
Gonzalez-Barrio, David
author
Xiao, Lihua
author
Carmena, David
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
King Saud University (Arabia Saudí)
2023-08-25T06:50:14Z
2023-08-25T06:50:14Z
2023-07
Front Vet Sci. 2023 Jul 6;10:1229151.
2297-1769
http://hdl.handle.net/20.500.12105/16338
37483300
10.3389/fvets.2023.1229151
2297-1769
Frontiers in veterinary science
Introduction: Domestic dogs and cats can be a source of human infection by a wide diversity of zoonotic pathogens including parasites. Genotyping and subtyping tools are useful in assessing the true public health relevance of canine and feline infections by these pathogens. This study investigated the occurrence, genetic diversity, and zoonotic potential of common diarrhea-causing enteric protist parasites in household dogs and cats in Egypt, a country where this information is particularly scarce. Methods: In this prospective, cross-sectional study a total of 352 individual fecal samples were collected from dogs (n = 218) and cats (n = 134) in three Egyptian governorates (Dakahlia, Gharbeya, and Giza) during July-December 2021. Detection and identification of Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, and Blastocystis sp. were carried out by PCR and Sanger sequencing. Basic epidemiological variables (geographical origin, sex, age, and breed) were examined for association with occurrence of infection by enteric protists. Results and discussion: The overall prevalence rates of Cryptosporidium spp. and G. duodenalis were 1.8% (95% CI: 0.5-4.6) and 38.5% (95% CI: 32.0-45.3), respectively, in dogs, and 6.0% (95% CI: 2.6-11.4) and 32.1% (95% CI: 24.3-40.7), respectively, in cats. All canine and feline fecal samples analyzed tested negative for E. bieneusi and Blastocystis sp. Dogs from Giza governorate and cats from Dakahlia governorate were at higher risk of infection by Cryptosporidium spp. (p = 0.0006) and G. duodenalis (p = 0.00001), respectively. Sequence analyses identified host-adapted Cryptosporidium canis (n = 4, one of them belonging to novel subtype XXe2) and G. duodenalis assemblages C (n = 1) and D (n = 3) in dogs. In cats the zoonotic C. parvum (n = 5) was more prevalent than host-adapted C. felis (n = 1). Household dogs had a limited (but not negligible) role as source of human giardiasis and cryptosporidiosis, but the unexpected high frequency of zoonotic C. parvum in domestic cats might be a public health concern. This is the first molecular-based description of Cryptosporidium spp. infections in cats in the African continent to date. Molecular epidemiological data provided here can assist health authorities and policy makers in designing and implementing effective campaigns to minimize the transmission of enteric protists in Egypt.
eng
Enteric parasites
Epidemiology
Zoonoses
Genotyping
Small subunit ribosomal RNA gene
60 kDa glycoprotein
Molecular characteristics and zoonotic potential of enteric protists in domestic dogs and cats in Egypt
research article
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