2026-04-29T06:16:00Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/163382024-09-27T22:36:48Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Elmahallawy, Ehab Kotb author Gareh, Ahmed author Abu-Okail, Akram author Köster, Pamela Carolina author Dashti, Alejandro author Asseri, Jamal author Gouda, Asmaa Aboelabbas author Mubaraki, Murad A author Mohamed, Sara Abdel-Aal author Mohamed, Yasser M author Hassan, Ehssan Ahmed author Elgendy, Mohamed author Hernández-Castro, Carolina author Bailo-Barroso, Begoña author Gonzalez-Barrio, David author Xiao, Lihua author Carmena, David author 2023-07 Introduction: Domestic dogs and cats can be a source of human infection by a wide diversity of zoonotic pathogens including parasites. Genotyping and subtyping tools are useful in assessing the true public health relevance of canine and feline infections by these pathogens. This study investigated the occurrence, genetic diversity, and zoonotic potential of common diarrhea-causing enteric protist parasites in household dogs and cats in Egypt, a country where this information is particularly scarce. Methods: In this prospective, cross-sectional study a total of 352 individual fecal samples were collected from dogs (n = 218) and cats (n = 134) in three Egyptian governorates (Dakahlia, Gharbeya, and Giza) during July-December 2021. Detection and identification of Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, and Blastocystis sp. were carried out by PCR and Sanger sequencing. Basic epidemiological variables (geographical origin, sex, age, and breed) were examined for association with occurrence of infection by enteric protists. Results and discussion: The overall prevalence rates of Cryptosporidium spp. and G. duodenalis were 1.8% (95% CI: 0.5-4.6) and 38.5% (95% CI: 32.0-45.3), respectively, in dogs, and 6.0% (95% CI: 2.6-11.4) and 32.1% (95% CI: 24.3-40.7), respectively, in cats. All canine and feline fecal samples analyzed tested negative for E. bieneusi and Blastocystis sp. Dogs from Giza governorate and cats from Dakahlia governorate were at higher risk of infection by Cryptosporidium spp. (p = 0.0006) and G. duodenalis (p = 0.00001), respectively. Sequence analyses identified host-adapted Cryptosporidium canis (n = 4, one of them belonging to novel subtype XXe2) and G. duodenalis assemblages C (n = 1) and D (n = 3) in dogs. In cats the zoonotic C. parvum (n = 5) was more prevalent than host-adapted C. felis (n = 1). Household dogs had a limited (but not negligible) role as source of human giardiasis and cryptosporidiosis, but the unexpected high frequency of zoonotic C. parvum in domestic cats might be a public health concern. This is the first molecular-based description of Cryptosporidium spp. infections in cats in the African continent to date. Molecular epidemiological data provided here can assist health authorities and policy makers in designing and implementing effective campaigns to minimize the transmission of enteric protists in Egypt. Front Vet Sci. 2023 Jul 6;10:1229151. 2297-1769 http://hdl.handle.net/20.500.12105/16338 37483300 10.3389/fvets.2023.1229151 2297-1769 Frontiers in veterinary science Enteric parasites Epidemiology Zoonoses Genotyping Small subunit ribosomal RNA gene 60 kDa glycoprotein Molecular characteristics and zoonotic potential of enteric protists in domestic dogs and cats in Egypt