2026-06-14T03:40:43Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/163082024-09-27T23:56:47Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Obando, Ignacio author Muñoz-Almagro, Carmen author Arroyo, Luis A author Tarrago Asensio, David author Sanchez-Tatay, David author Moreno-Perez, David author Dhillon, Sahar S author Esteva, Cristina author Hernandez-Bou, Susanna author Garcia-Garcia, Juan J author Hausdorff, William P author Brueggemann, Angela B author 2008-09 Pediatric parapneumonic empyema (PPE) has been increasing in several countries including Spain. Streptococcus pneumoniae is a major PPE pathogen; however, antimicrobial pretreatment before pleural fluid (PF) sampling frequently results in negative diagnostic cultures, thus greatly underestimating the contribution of pneumococci, especially pneumococci susceptible to antimicrobial agents, to PPE. The study aim was to identify the serotypes and genotypes that cause PPE by using molecular diagnostics and relate these data to disease incidence and severity. A total of 208 children with PPE were prospectively enrolled; blood and PF samples were collected. Pneumococci were detected in 79% of culture-positive and 84% of culture-negative samples. All pneumococci were genotyped by multilocus sequence typing. Serotypes were determined for 111 PPE cases; 48% were serotype 1, of 3 major genotypes previously circulating in Spain. Variance in patient complication rates was statistically significant by serotype. The recent PPE increase is principally due to nonvaccine serotypes, especially the highly invasive serotype 1. Emerg Infect Dis. 2008 Sep;14(9):1390-7. 10.3201/eid1409.071094 1080-6059 Emerging infectious diseases 18760005 http://hdl.handle.net/20.500.12105/16308 Pediatric parapneumonic empyema, Spain