2026-05-21T23:39:06Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/162852025-04-04T17:59:17Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc White, Ruby author Sotillo, Javier author Ancarola, María Eugenia author Borup, Anne author Boysen, Anders Toftegaard author Brindley, Paul J author Buzás, Edit I author Cavallero, Serena author Chaiyadet, Sujittra author Chalmers, Iain W author Cucher, Marcela A author Dagenais, Maude author Davis, Chelsea N author Devaney, Eileen author Duque-Correa, Maria A author Eichenberger, Ramon Marc author Fontenla, Santiago author Gasan, Thomas A author Hokke, Cornelis H author Kosanovic, Maja author Kuipers, Marije E author Laha, Thewarach author Loukas, Alex author Maizels, Rick M author Marcilla, Antonio author Mazanec, Hynek author Morphew, Russell M author Neophytou, Kyriaki author Nguyen, Linh Thuy author Nolte-'t Hoen, Esther author Povelones, Michael author Robinson, Mark W author Rojas, Alicia author Schabussova, Irma author Smits, Hermelijn H author Sungpradit, Sivapong author Tritten, Lucienne author Whitehead, Bradley author Zakeri, Amin author Nejsum, Peter author Buck, Amy H author Hoffmann, Karl F author 2023-01 Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms. These challenges include, but are not limited to, generating sufficient quantities of EVs for descriptive or functional studies, defining pan-helminth EV markers, genetically modifying these organisms, and identifying rigorous methodologies for in vitro and in vivo studies. Here, we outline best practices for those investigating the biology of helminth-derived EVs to complement the MISEV guidelines. We summarise community-agreed standards for studying EVs derived from this broad set of non-model organisms, raise awareness of issues associated with helminth EVs and provide future perspectives for how progress in the field will be achieved. J Extracell Vesicles. 2023 Jan;12(1):e12298. 10.1002/jev2.12298 2001-3078 Journal of extracellular vesicles 36604533 http://hdl.handle.net/20.500.12105/16285 EV guidelines EV reporting Electron microscopy Extracellular vesicles Helminths Parasites Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility