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oai:repisalud.isciii.es:20.500.12105/162782024-09-27T09:53:33Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Del Monte-Monge, Alberto author Martos-Folgado, Inmaculada author Rodríguez-Ronchel, Ana author Ramiro, Almudena R author 2023-04-30 The antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-derived antibodies. We describe steps for bone marrow transfer from donor mice into irradiated recipient mice. We then detail immunization of mouse chimeras with atheroprotective malondialdehyde low-density lipoprotein during high-fat diet feeding and atherosclerosis burden analysis. For complete details on the use and execution of this protocol, please refer to Martos-Folgado et al. (2022).1. STAR Protoc. 2023 Apr 30;4(2):102274 10.1016/j.xpro.2023.102274 2666-1667 STAR protocols 37126444 http://hdl.handle.net/20.500.12105/16278 Assessing the impact of an antigen-specific antibody response on atherosclerosis development in mice.