2026-04-28T22:57:09Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/162662025-04-30T12:39:08Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Maloney, Jenny G
author
Molokin, Aleksey
author
Seguí, Raimundo
author
Maravilla, Pablo
author
Martínez-Hernández, Fernando
author
Villalobos, Guiehdani
author
Tsaousis, Anastasios D
author
Gentekaki, Eleni
author
Muñoz-Antoli, Carla
author
Klisiowicz, Debora R
author
Oishi, Camila Y
author
Toledo, Rafael
author
Esteban, J Guillermo
author
Köster, Pamela Carolina
author
de Lucio, Aida
author
Dashti, Alejandro
author
Bailo-Barroso, Begoña
author
Calero-Bernal, Rafael
author
Gonzalez-Barrio, David
author
Carmena, David
author
Santín, Mónica
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
United States Department of Agriculture. Agricultural Research Service
2023-07-17T10:35:27Z
2023-07-17T10:35:27Z
2023
Microorganisms. 2023;11(1):46.
2076-2607
http://hdl.handle.net/20.500.12105/16266
36677338
10.3390/microorganisms11010046
Microorganisms
Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity.
eng
Blastocystis
B. lapemi
Subtype
Oxford Nanopore MinION
ssu rRNA
Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38
research article
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URL
https://repisalud.isciii.es/bitstreams/cbdd0255-28cc-4e51-a7ae-f055c6f8a081/download
File
MD5
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application/pdf
IdentificationMolecularCharacterizationFour_2023.pdf
URL
https://repisalud.isciii.es/bitstreams/cf846392-4cde-4dae-9f9e-6b33190b0906/download
File
MD5
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text/plain
IdentificationMolecularCharacterizationFour_2023.pdf.txt