2026-04-28T22:57:08Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/162662025-04-30T12:39:08Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Maloney, Jenny G author Molokin, Aleksey author Seguí, Raimundo author Maravilla, Pablo author Martínez-Hernández, Fernando author Villalobos, Guiehdani author Tsaousis, Anastasios D author Gentekaki, Eleni author Muñoz-Antoli, Carla author Klisiowicz, Debora R author Oishi, Camila Y author Toledo, Rafael author Esteban, J Guillermo author Köster, Pamela Carolina author de Lucio, Aida author Dashti, Alejandro author Bailo-Barroso, Begoña author Calero-Bernal, Rafael author Gonzalez-Barrio, David author Carmena, David author Santín, Mónica author 2023 Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity. Microorganisms. 2023;11(1):46. 2076-2607 http://hdl.handle.net/20.500.12105/16266 36677338 10.3390/microorganisms11010046 Microorganisms Blastocystis B. lapemi Subtype Oxford Nanopore MinION ssu rRNA Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38