2026-04-29T19:35:01Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/162552024-09-27T21:09:34Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Chopra, Kunal
author
Folkmanaitė, Milda
author
Stockdale, Liam
author
Shathish, Vishali
author
Ishibashi, Shoko
author
Bergin, Rachel
author
Amich, Jorge
author
Amaya, Enrique
funder
The Scar Free Foundation
funder
British Heart Foundation
funder
Medical Research Council (Reino Unido)
2023-07-17T08:37:05Z
2023-07-17T08:37:05Z
2023-03-17
iScience. 2023 Feb 4;26(3):106147.
10.1016/j.isci.2023.106147
2589-0042
iScience
36843843
http://hdl.handle.net/20.500.12105/16255
Sustained elevated levels of reactive oxygen species (ROS) have been shown to be essential for regeneration in many organisms. This has been shown primarily via the use of pharmacological inhibitors targeting the family of NADPH oxidases (NOXes). To identify the specific NOXes involved in ROS production during adult caudal fin regeneration in zebrafish, we generated nox mutants for duox, nox5 and cyba (a key subunit of NOXes 1-4) and crossed these lines with a transgenic line ubiquitously expressing HyPer, which permits the measurement of ROS levels. Homozygous duox mutants had the greatest effect on ROS levels and rate of fin regeneration among the single mutants. However, duox:cyba double mutants showed a greater effect on fin regeneration than the single duox mutants, suggesting that Nox1-4 also play a role during regeneration. This work also serendipitously found that ROS levels in amputated adult zebrafish fins oscillate with a circadian rhythm.
eng
Cell biology
Ichthyology
Molecular genetics
Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
research article
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DuoxPrimaryNADPHOxidaseResponsible_2023.pdf
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