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oai:repisalud.isciii.es:20.500.12105/162552024-09-27T21:09:34Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Chopra, Kunal author Folkmanaitė, Milda author Stockdale, Liam author Shathish, Vishali author Ishibashi, Shoko author Bergin, Rachel author Amich, Jorge author Amaya, Enrique author 2023-03-17 Sustained elevated levels of reactive oxygen species (ROS) have been shown to be essential for regeneration in many organisms. This has been shown primarily via the use of pharmacological inhibitors targeting the family of NADPH oxidases (NOXes). To identify the specific NOXes involved in ROS production during adult caudal fin regeneration in zebrafish, we generated nox mutants for duox, nox5 and cyba (a key subunit of NOXes 1-4) and crossed these lines with a transgenic line ubiquitously expressing HyPer, which permits the measurement of ROS levels. Homozygous duox mutants had the greatest effect on ROS levels and rate of fin regeneration among the single mutants. However, duox:cyba double mutants showed a greater effect on fin regeneration than the single duox mutants, suggesting that Nox1-4 also play a role during regeneration. This work also serendipitously found that ROS levels in amputated adult zebrafish fins oscillate with a circadian rhythm. iScience. 2023 Feb 4;26(3):106147. 10.1016/j.isci.2023.106147 2589-0042 iScience 36843843 http://hdl.handle.net/20.500.12105/16255 Cell biology Ichthyology Molecular genetics Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish