2026-07-21T15:57:15Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/161922025-05-12T08:44:16Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19610

00925njm 22002777a 4500 dc González-Rubio, Juana María author Domínguez-Morueco, Noelia author Pedraza-Diaz, Susana author Cañas Portilla, Ana Isabel author Lucena-Lozano, Miguel Angel author Rodriguez, Ana author Castaño, Argelia author Esteban-Lopez, Marta author 2023-07 Human exposure to mercury can have serious health effects, especially in vulnerable groups such as children and fetuses. The use of dried blood spot (DBS) samples to collect capillary blood greatly facilitates sample collection and fieldwork, being a less invasive alternative to blood collection by venipuncture, needing a small volume of sample, and does not require specialized medical staff. Moreover, DBS sampling reduces logistical and financial barriers related to transport and storage of blood samples. We propose here a novel method to analyze total mercury in DBS samples in a Direct Mercury Analyzer (DMA) that allow the control of the volume of the DBS samples. This method has shown good results in terms of precision (<6% error), accuracy (<10% coefficient of variation) and recovery (75-106%). The applicability of the method in human biomonitoring (HBM) was demonstrated in a pilot study involving 41 adults aged 18-65. Mercury concentrations of DBS samples from capillary blood collected by finger prick (real DBS samples) were determined in the DMA and compared with those determined in whole blood (venous blood) by ICP-MS, the method usually used in HBM. The sampling procedure was also validated by comparison of real DBS samples and DBS generated artificially in the laboratory by depositing venous samples in cellulose cards (laboratory DBS). There were no statistically significant differences in the results obtained using both methodologies (DMA: Geometric Mean (confidence interval 95%) = 3.87 (3.12-4.79) µg/L; ICP-MS: Geometric Mean (confidence interval 95%) = 3.46 (2.80-4.27) µg/L). The proposed method is an excellent alternative to be applied in clinical settings as screening methodology for assessing mercury exposure in vulnerable groups, such us pregnant woman, babies and children. Environ Int. 2023 Jul;177:107958. 10.1016/j.envint.2023.107958 1873-6750 Environment international 37285712 http://hdl.handle.net/20.500.12105/16192 Direct Mercury Analyser (DMA) Dried Blood Spot (DBS) HBM4EU Human Biomonitoring Mercury Microsampling Non-invasive A simple method for direct mercury analysis in dried blood spots (DBS) samples for human biomonitoring studies