2026-06-14T03:54:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/161602025-06-24T16:11:09Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19608col_20.500.12105_19609
Repisalud
author
Jacqueline Forget, Camille
author
Gavilán, Ana M
author
Lopez-Perea, Noemi
author
Penedos, Ana Raquel
author
Masa-Calles, Josefa
author
Echevarria, Juan Emilio
author
Fernandez-Garcia, Aurora
author
MMR Study Group
funder
Instituto de Salud Carlos III
funder
Centro de Investigación Biomédica en Red - CIBERESP (Epidemiología y Salud Pública)
funder
Unión Europea. European Centre for Disease Prevention and Control (ECDC)
2023-06-13T07:20:38Z
2023-06-13T07:20:38Z
2023-05
Front Microbiol. 2023 May 22;14:1143933.
10.3389/fmicb.2023.1143933
1664-302X
Frontiers in microbiology
37283922
http://hdl.handle.net/20.500.12105/16160
Background: In countries entering the post-elimination phase for measles, the study of variants by sequencing of 450 nucleotides of the N gene (N450) does not always allow the tracing of chains of transmission. Indeed, between 2017 and 2020, most measles virus sequences belonged to either the MVs/Dublin.IRL/8.16 (B3-Dublin) or the MVs/Gir Somnath.IND/42.16 (D8-Gir Somnath) variants. We evaluated the additional use of a non-coding region (MF-NCR) as a tool to enhance resolution and infer case origin, chains of transmission and characterize outbreaks. Methods: We obtained 115 high-quality MF-NCR sequences from strains collected from Spanish patients infected with either B3-Dublin or D8-Gir Somnath variants between 2017 and 2020, performed epidemiological, phylogenetic and phylodynamic analyses and applied a mathematical model to determine relatedness among identified clades. Results: Applying this model allowed us to identify phylogenetic clades potentially derived from concomitant importations of the virus rather than single chain of transmission, inferred based on only N450 and epidemiology data. In a third outbreak, we found two related clades that corresponded to two chains of transmission. Discussion: Our results show the ability of the proposed method to improve identification of simultaneous importations in the same region which could trigger enhanced contact tracing. Moreover, the identification of further transmission chains indicates that the size of import-related outbreaks was smaller than previously found, supporting the interpretation that endemic measles transmission was absent in Spain between 2017 and 2020. We suggest considering the use of the MF-NCR region in conjunction with the study of N450 variants in future WHO recommendations for measles surveillance.
eng
B3 genotype
D8 genotype
Mathematical model
Outbreaks
Phylodynamic analyses
Phylogenetic analysis
Utility of MF-non coding region for measles molecular surveillance during post-elimination phase, Spain, 2017-2020
research article
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UtilityMF-nonCodingRegion_2023.pdf.txt
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