2026-06-14T03:31:48Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/161432024-09-27T21:00:09Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc García-Murria, Mª Jesús author Gadea-Salom, Laura author Moreno, Sandra author Rius-Salvador, Marina author Zaragoza, Oscar author Brun, Alejandro author Mingarro, Ismael author Martínez-Gil, Luis author 2023-05-24 Several approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2. Virol J. 2023 May 24;20(1):99. 10.1186/s12985-023-02068-1 1743-422X Virology journal 37226231 http://hdl.handle.net/20.500.12105/16143 Enveloped virus HTS High-throughput screening Influenza a virus Membrane fusion Nipah virus SARS-CoV-2 Syncytium Viral entry Identification of small molecules capable of enhancing viral membrane fusion