2026-06-14T03:44:03Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/161162025-06-13T09:33:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_19616col_20.500.12105_19617
Repisalud
author
Pérez-Montero, Andrea
author
Zaragoza, Oscar
author
Luque, Alfonso
author
Hortelano, Sonsoles
author
Acebo, Paloma
funder
Instituto de Salud Carlos III
funder
Comunidad de Madrid (España)
2023-05-24T08:39:32Z
2023-05-24T08:39:32Z
2023-03-05
Bio Protoc. 2023 Mar 5;13(5):e4629.
10.21769/BioProtoc.4629
2331-8325
Bio-protocol
36908642
http://hdl.handle.net/20.500.12105/16116
Lipid droplets (LD), triglycerides and sterol esters among them, are well known for their capacity as lipid storage organelles. Recently, they have emerged as critical cytoplasmic structures involved in numerous biological functions. LD storage is generated de novo by the cell and provides an energy reserve, lipid precursors, and cell protection. Moreover, LD accumulation can be observed in some pathologies as obesity, atherosclerosis, or lung diseases. Fluorescence imaging techniques are the most widely used techniques to visualize cellular compartments in live cells, including LD. Nevertheless, presence of fluorophores can damage subcellular components and induce cytotoxicity, or even alter the dynamics of the organelles. As an alternative to fluorescence microscopy, label-free techniques such as stimulated Raman scattering and coherent anti-stokes Raman scattering microscopy offer a solution to avoid the undesirable effects caused by dyes and fluorescent proteins, but are expensive and complex. Here, we describe a label-free method using live-cell imaging by 3D holotomographic microscopy (Nanolive) to visualize LD accumulation in the MH-S alveolar macrophage cell line after treatment with oleic acid, a monounsaturated fatty acid that promotes lipid accumulation.
eng
3D holotomographic microscopy
Alveolar macrophages
Lipid droplet
Lung pathologies
Metabolism
Oleic acid
Visualization of Lipid Droplets in the Alveolar Macrophage Cell Line MH-S with Live-cell Imaging by 3D Holotomographic Microscopy (Nanolive)
research article
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URL
https://repisalud.isciii.es/bitstreams/fc850b2d-8970-4a27-b929-4e97ba016ac7/download
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application/pdf
VisualizationLipidDropletsAlveolar_2023 .pdf
URL
https://repisalud.isciii.es/bitstreams/9898bbe5-fba9-4978-a261-61b215c20be5/download
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text/plain
VisualizationLipidDropletsAlveolar_2023 .pdf.txt