2026-06-14T05:05:17Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/160972025-06-24T17:51:35Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19610col_20.500.12105_19617
Repisalud
author
Torres-Ruiz, Mónica
author
De Alba-González, Mercedes
author
Morales, Mónica
author
Martin-Folgar, Raquel
author
González-Caballero, MCarmen
author
Cañas Portilla, Ana Isabel
author
De la Vieja, Antonio
funder
Ministerio de Ciencia e Innovación (España)
funder
Agencia Estatal de Investigación (España)
funder
Instituto de Salud Carlos III
funder
National University of Distance Education (España)
2023-05-18T12:30:52Z
2023-05-18T12:30:52Z
2023-05-20
Sci Total Environ. 2023 May 20;874:162406.
10.1016/j.scitotenv.2023.162406
1879-1026
The Science of the total environment
36841402
http://hdl.handle.net/20.500.12105/16097
Nanoplastics (NP) are present in aquatic and terrestrial ecosystems. Humans can be exposed to them through contaminated water, food, air, or personal care products. Mechanisms of NP toxicity are largely unknown and the Zebrafish embryo poses an ideal model to investigate them due to its high homology with humans. Our objective in the present study was to combine a battery of behavioral assays with the study of endocrine related gene expression, to further explore potential NP neurotoxic effects on animal behavior. Polystyrene nanoplastics (PSNP) were used to evaluate NP toxicity. Our neurobehavioral profiles include a tail coiling assay, a light/dark activity assay, two thigmotaxis anxiety assays (auditory and visual stimuli), and a startle response - habituation assay in response to auditory stimuli. Results show PSNP accumulated in eyes, neuromasts, brain, and digestive system organs. PSNP inhibited acetylcholinesterase and altered endocrine-related gene expression profiles both in the thyroid and glucocorticoid axes. At the whole organism level, we observed altered behaviors such as increased activity and anxiety at lower doses and lethargy at a higher dose, which could be due to a variety of complex mechanisms ranging from sensory organ and central nervous system effects to others such as hormonal imbalances. In addition, we present a hypothetical adverse outcome pathway related to these effects. In conclusion, this study provides new understanding into NP toxic effects on zebrafish embryo, emphasizing a critical role of endocrine disruption in observed neurotoxic behavioral effects, and improving our understanding of their potential health risks to human populations.
eng
Behavior
Development
Hormones
Nanomaterial
Plastic
Toxicity
Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo
research article
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NeurotoxicityEndocrineDisruptionCaused_2023.pdf
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NeurotoxicityEndocrineDisruptionCaused_2023.pdf.txt