2026-06-14T05:06:29Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/160972025-06-24T17:51:35Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19610col_20.500.12105_19617

00925njm 22002777a 4500 dc Torres-Ruiz, Mónica author De Alba-González, Mercedes author Morales, Mónica author Martin-Folgar, Raquel author González-Caballero, MCarmen author Cañas Portilla, Ana Isabel author De la Vieja, Antonio author 2023-05-20 Nanoplastics (NP) are present in aquatic and terrestrial ecosystems. Humans can be exposed to them through contaminated water, food, air, or personal care products. Mechanisms of NP toxicity are largely unknown and the Zebrafish embryo poses an ideal model to investigate them due to its high homology with humans. Our objective in the present study was to combine a battery of behavioral assays with the study of endocrine related gene expression, to further explore potential NP neurotoxic effects on animal behavior. Polystyrene nanoplastics (PSNP) were used to evaluate NP toxicity. Our neurobehavioral profiles include a tail coiling assay, a light/dark activity assay, two thigmotaxis anxiety assays (auditory and visual stimuli), and a startle response - habituation assay in response to auditory stimuli. Results show PSNP accumulated in eyes, neuromasts, brain, and digestive system organs. PSNP inhibited acetylcholinesterase and altered endocrine-related gene expression profiles both in the thyroid and glucocorticoid axes. At the whole organism level, we observed altered behaviors such as increased activity and anxiety at lower doses and lethargy at a higher dose, which could be due to a variety of complex mechanisms ranging from sensory organ and central nervous system effects to others such as hormonal imbalances. In addition, we present a hypothetical adverse outcome pathway related to these effects. In conclusion, this study provides new understanding into NP toxic effects on zebrafish embryo, emphasizing a critical role of endocrine disruption in observed neurotoxic behavioral effects, and improving our understanding of their potential health risks to human populations. Sci Total Environ. 2023 May 20;874:162406. 10.1016/j.scitotenv.2023.162406 1879-1026 The Science of the total environment 36841402 http://hdl.handle.net/20.500.12105/16097 Behavior Development Hormones Nanomaterial Plastic Toxicity Neurotoxicity and endocrine disruption caused by polystyrene nanoparticles in zebrafish embryo