2026-04-29T07:24:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/160862024-09-27T21:39:30Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Villalón, Pilar
author
Bárcena, Marta
author
Medina-Pascual, Maria Jose
author
Garrido, Noelia
author
Pino-Rosa, Silvia del
author
Carrasco, Gema
author
Valdezate, Sylvia
funder
Instituto de Salud Carlos III
2023-05-17T12:58:52Z
2023-05-17T12:58:52Z
2023-01-06
Antibiotics (Basel). 2023 Jan 6;12(1):99.
2079-6382
http://hdl.handle.net/20.500.12105/16086
36671301
10.3390/antibiotics12010099
Antibiotics (Basel, Switzerland)
Background: This work reports on antimicrobial resistance data for invasive Streptococcus pyogenes in Spain, collected by the 'Surveillance Program for Invasive Group A Streptococcus', in 2007-2020. Methods: emm typing was determined by sequencing. Susceptibility to penicillin, tetracycline, erythromycin, and clindamycin was determined via the E-test. tetM, tetO, msrD, mefA, ermB, ermTR, and ermT were sought by PCR. Macrolide-resistant phenotypes (M, cMLSB, and iMLSB) were detected using the erythromycin-clindamycin double-disk test. Resistant clones were identified via their emm type, multilocus sequence type (ST), resistance genotype, and macrolide resistance phenotype. Results: Penicillin susceptibility was universal. Tetracycline resistance was recorded for 237/1983 isolates (12.0%) (152 carried only tetM, 48 carried only tetO, and 33 carried both). Erythromycin resistance was detected in 172/1983 isolates (8.7%); ermB was present in 83, mefA in 58, msrD in 51, ermTR in 46, and ermT in 36. Clindamycin resistance (methylase-mediated) was present in 78/1983 isolates (3.9%). Eight main resistant clones were identified: two that were tetracycline-resistant only (emm22/ST46/tetM and emm77/ST63/tetO), three that were erythromycin-resistant only (emm4/ST39/mefA-msrD/M, emm12/ST36/mefA-msrD/M, and emm28/ST52/ermB/cMLSB), and three that were tetracycline-erythromycin co-resistant (emm11/ST403/tetM-ermB/cMLSB, emm77/ST63/tetO-ermTR/iMLSB, and emm77/ST63/tetM-tetO-ermTR/iMLSB). Conclusions: Tetracycline, erythromycin, and clindamycin resistance rates declined between 2007 and 2020. Temporal variations in the proportion of resistant clones determined the change in resistance rates.
eng
Streptococcus pyogenes
Antimicrobial resistance
Clindamycin
Clone
Emm type
Erythromycin
Genotype
Phenotype
Tetracycline
National Surveillance of Tetracycline, Erythromycin, and Clindamycin Resistance in Invasive Streptococcus pyogenes: A Retrospective Study of the Situation in Spain, 2007-2020
journal article
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https://repisalud.isciii.es/bitstreams/42a5e620-ab8c-47ef-8633-817a67a51be2/download
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application/pdf
NationalSurveillanceTetracyclineErythromycin_2023.pdf
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https://repisalud.isciii.es/bitstreams/6078b76b-3f7b-4a56-96d1-12efd48fb91c/download
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NationalSurveillanceTetracyclineErythromycin_2023.pdf.txt