2026-08-05T11:38:44Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/160812024-09-27T21:53:47Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Penela-Sánchez, Daniel
author
Rocafort, Muntsa
author
Henares, Desiree
author
Jordan, Iolanda
author
Brotons, Pedro
author
Cabrerizo, Maria
author
Launes, Cristian
author
Muñoz-Almagro, Carmen
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2023-05-17T11:19:16Z
2023-05-17T11:19:16Z
2023-06
Pediatr Pulmonol. 2023 Jun;58(6):1728-1737.
10.1002/ppul.26393
1099-0496
Pediatric pulmonology
36988404
http://hdl.handle.net/20.500.12105/16081
Introduction: Rhinoviruses (RV) and enteroviruses (EV) are among the main causative etiologies of lower respiratory tract infection (LRTI) in children. The clinical spectrum of RV/EV infection is wide, which could be explained by diverse environmental, pathogen-, and host-related factors. Little is known about the nasopharyngeal microbiota as a risk factor or disease modifier for RV/EV infection in pediatric patients. This study describes distinct nasopharyngeal microbiota profiles according to RV/EV LRTI status in children. Methods: Cross-sectional case-control study, conducted at Hospital Sant de Déu (Barcelona, Spain) from 2017 to 2020. Three groups of children <5 years were included: healthy controls without viral detection (Group A), mild or asymptomatic controls with RV/EV infection (Group B), and cases with severe RV/EV infection admitted to the pediatric intensive care unit (PICU) (Group C). Nasopharyngeal samples were collected from participants for viral DNA/RNA detection by multiplex-polymerase chain reaction and bacterial microbiota characterization by 16S rRNA gene sequencing. Results: A total of 104 subjects were recruited (A = 17, B = 34, C = 53). Children's nasopharyngeal microbiota composition varied according to their RV/EV infection status. Richness and diversity were decreased among children with severe infection. Nasopharyngeal microbiota profiles enriched in genus Dolosigranulum were related to respiratory health, while genus Haemophilus was specifically predominant in children with severe RV/EV LRTI. Children with mild or asymptomatic RV/EV infection showed an intermediate profile. Conclusions: These results suggest a close relationship between the nasopharyngeal microbiota and different clinical presentations of RV/EV infection.
eng
Enterovirus
Intensive care units
Lower respiratory tract infection
Microbiota
Rhinovirus
Impact of the bacterial nasopharyngeal microbiota on the severity of genus enterovirus lower respiratory tract infection in children: A case-control study
journal article
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ImpactBacterialNasopharyngealMicrobiota_2023.pdf
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ImpactBacterialNasopharyngealMicrobiota_2023.pdf.txt