2026-06-14T03:42:21Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/160802024-10-31T11:55:33Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Tijani, Muyideen K
author
Köster, Pamela Carolina
author
Guadano-Procesi, Isabel
author
George, Imo S
author
Abodunrin, Elizabeth
author
Adeola, Adedamola
author
Dashti, Alejandro
author
Bailo-Barroso, Begoña
author
Gonzalez-Barrio, David
author
Carmena, David
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
2023-05-17T11:10:00Z
2023-05-17T11:10:00Z
2023-02-28
Trop Med Infect Dis. 2023 Feb 28;8(3):152.
10.3390/tropicalmed8030152
2414-6366
Tropical medicine and infectious disease
36977153
http://hdl.handle.net/20.500.12105/16080
Giardia duodenalis is a significant contributor to the burden of diarrheal disease in sub-Saharan Africa. This study assesses the occurrence and molecular diversity of G. duodenalis and other intestinal parasites in apparently healthy children (n = 311) in Ibadan, Nigeria. Microscopy was used as a screening method and PCR and Sanger sequencing as confirmatory and genotyping methods, respectively. Haplotype analyses were performed to examine associations between genetic variants and epidemiological variables. At microscopy examination, G. duodenalis was the most prevalent parasite found (29.3%, 91/311; 95% CI: 24.3-34.7), followed by Entamoeba spp. (18.7%, 58/311; 14.5-23.4), Ascaris lumbricoides (1.3%, 4/311; 0.4-3.3), and Taenia sp. (0.3%, 1/311; 0.01-1.8). qPCR confirmed the presence of G. duodenalis in 76.9% (70/91) of the microscopy-positive samples. Of them, 65.9% (60/91) were successfully genotyped. Assemblage B (68.3%, 41/60) was more prevalent than assemblage A (28.3%, 17/60). Mixed A + B infections were identified in two samples (3.3%, 2/60). These facts, together with the absence of animal-adapted assemblages, suggest that human transmission of giardiasis was primarily anthroponotic. Efforts to control G. duodenalis (and other fecal-orally transmitted pathogens) should focus on providing safe drinking water and improving sanitation and personal hygiene practices.
eng
Africa
Diarrhea
Epidemiology
Giardiasis
Intestinal parasites
Transmission
High Diversity of Giardia duodenalis Assemblages and Sub-Assemblages in Asymptomatic School Children in Ibadan, Nigeria
journal article
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