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oai:repisalud.isciii.es:20.500.12105/160512024-11-28T16:30:01Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16938col_20.500.12105_16963

00925njm 22002777a 4500 dc Lara-Aguilar, Violeta author Valle-Millares, Daniel author Crespo-Bermejo, Celia author Grande-García, Sergio author Llamas-Adán, Manuel author Cortijo-Alfonso, María Engracia author Martín-Carbonero, Luz author Domínguez, Lourdes author Ryan, Pablo author de Los Santos, Ignacio author Bartolomé-Sánchez, Sofía author Vidal-Alcántara, Erick Joan author Jimenez-Sousa, Maria Angeles author Fernandez-Rodriguez, Amanda author Briz, Veronica author Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP) author 2023-06 Background: We identified that acute or chronic Hepatitis C (HCV) infection in people living with HIV (PLWHIV) results in different senescence profiles. However, variations in these profiles after HCV elimination, spontaneously or with direct-acting antivirals (DAAs), remain unclear. Methods: Longitudinal observational study (48 weeks) in 70 PLWHIV: 23 PLWHIV with active HCV-chronic infection (CHC) before and after HCV eradication with DAAs, 12 PLWHIV who spontaneously clarify the HCV (SC), and 35 controls (HIV). Oxidative stress was quantified at DNA, lipid, protein, and nitrate levels, as well as the antioxidant capacity and glutathione enzyme. The replicative senescence was evaluated by relative telomere length measurement by PCR and twenty-six factors related to Senescence-Associated Secretory Phenotype (SASP) were characterized by Luminex. Differences in senescence markers was evaluated by generalized linear models. Results: During follow-up, the SC group achieved a significant improvement in glutathione enzyme and lipid peroxidation. The secretion of SASP markers increased but was still lower than that of the HIV group. Overall, the CHC group reduced the levels of oxidative stress and SASP markers to levels like those of the HIV group. No significant differences in telomere shortening were observed between groups. Conclusions: As the time since spontaneous resolution of HCV infection increased, patients had an improved senescence profile compared to the HIV group. Elimination of chronic HCV infection by DAAs led to a partial improvement of the senescent profile by restoring oxidative stress levels. However, although some SASP markers reached levels like those of the HIV group, others remained altered. Biomed Pharmacother. 2023 Jun;162:114664. 10.1016/j.biopha.2023.114664 1950-6007 Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 37031491 http://hdl.handle.net/20.500.12105/16051 Ageing DAAs HCV/HIV Oxidative stress SASP Dynamics of cellular senescence markers after HCV elimination spontaneously or by DAAs in people living with HIV