2026-06-14T03:40:34Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/160482024-09-27T23:34:55Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Ayuso-Sáinz, Juan J.
author
Belinchón-Lorenzo, Silvia
author
Fernández-Cotrina, Javier
author
Jimenez, Maribel
author
Orduña-Domingo, Antonio
author
Quinto, Emiliano J
2023-05-10T10:39:54Z
2023-05-10T10:39:54Z
2022
Med. Weter. 2022,78(2):91-94.
10.21521/mw.6615
0025-8628
Medycyna Weterynaryjna
http://hdl.handle.net/20.500.12105/16048
The study of potential wild mammal reservoirs is necessary for the surveillance of leishmaniosis, as Leishmania protozoans have been isolated from a wide range of wild and domestic animal species, including Leporidae. Recently, it has been demonstrated that both hares and wild rabbits can act as sylvatic reservoirs of Leishmania. In Spain, most of the research involving wild rabbits has been developed in the central area of Madrid and in the southeastern Mediterranean coast. We studied the presence of Leishmania infantum in 116 wild rabbits (Oryctolagus cuniculus) captured in Santovenia de Pisuerga, Valladolid, Spain. Hair samples were analyzed by real time PCR. L. infantum kinetoplast DNA (kDNA) was detected and quantified in 4 out of 116 analyzed animals. The estimated number of parasites obtained were quite variable, ranging from 2.60 to 276.60. Hair samples can be collected by non-invasive methods, being a proper sample for Leishmania detection in wild Leporidae, which have an important role as reservoirs of Leishmania. Our findings enhance the need for more extensive studies in different geographical areas.
eng
Hair
Leishmania infantum
Rabbit
kDNA
Real Time PCR
Detection of Leishmania infantum kinetoplast DNA by Real Time PCR in hair of wild rabbits
journal article
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URL
https://repisalud.isciii.es/bitstreams/e0475e04-2b6c-4779-8ad2-0f421d04b4e5/download
File
MD5
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application/pdf
DetectionLeishmaniaInfantumKinetoplast_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/92e57fb3-0a2a-4d27-871c-1c7b8f0db310/download
File
MD5
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text/plain
DetectionLeishmaniaInfantumKinetoplast_2022.pdf.txt