2026-06-14T03:53:29Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/160252025-05-05T10:20:42Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16958
Repisalud
author
Pérez-Palacios, Patricia
author
Gual-de-Torrella, Ana
author
Delgado-Valverde, Mercedes
author
Oteo-Iglesias, Jesus
author
Hidalgo-Díaz, Carmen
author
Pascual, Álvaro
author
Fernández-Cuenca, Felipe
funder
Ministerio de Sanidad y Consumo (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2023-05-09T08:07:11Z
2023-05-09T08:07:11Z
2022-01
Microbiol Res. 2022 Jan;254:126894.
10.1016/j.micres.2021.126894
1618-0623
Microbiological research
34717138
http://hdl.handle.net/20.500.12105/16025
The spread of OXA-48-encoding plasmids from Klebsiella pneumoniae (OXA-48-Kpn), especially successful high-risk (HR) clones, is a growing concern. Biofilm formation can contribute to the dissemination of OXA-48-Kpn. It is not known whether biocides can affect the transfer of OXA-48-Kpn in biofilm. The aim of this study was to evaluate the effect of biocides on the conjugation frequency (CF) of OXA-48-Kpn in both biofilm and planktonic cultures. For that, seven OXA-48-Kpn isolates (4 belonging to HR clones and 3 to non-HR clones) were selected as donors. Each isolate was mixed (1:1) with Escherichia coli J53 (recipient) and grown on polystyrene microplates without biocides (control) and with 0.25x MIC of triclosan (TRI), chlorhexidine digluconate (CHX), povidone-iodine (POV), sodium hypochlorite (SOD) or ethanol (ETH). The CF was calculated as the number of transconjugants/number of E. coli J53. The results showed that for isolates growing in the absence of biocide, the mean fold change in the CF in biofilm with respect to that determined in planktonic cells (CF-BF/CF-PK) was 0.2 in non-HR isolates and ranged from 2.0 to 14.7 in HR isolates. In HR isolates grown in the presence of biocide, especially CHX, TRI, and ETH, the fold changes in CF-BF/CF-PK decreased, whereas in non-HR isolates the fold changes were similar or increased slightly with CHX, ETH, SOD and POV. In conclusion, the fold changes in the CF-BF/CF-PK are higher in HR isolates comparing to non-HR isolates in abscence of biocides. The fold changes in CF-BF/CF-PK of the HR isolates in the presence of biocides varied with the type of biocides, whereas in non-HR isolates, biocides have no significant effect, or produce only a slight increase in the fold change of CF-BF/CF-PK.
eng
Biocides
Biofilm
Carbapenemase-producing
K. pneumoniae
Plasmid conjugation
Transfer of plasmids harbouring blaOXA-48-like carbapenemase genes in biofilm-growing Klebsiella pneumoniae: Effect of biocide exposure
research article
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URL
https://repisalud.isciii.es/bitstreams/9fb06988-2ecb-403e-8589-cbf3afe11705/download
File
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35547e3a5dbcf6b35a58655b63973ba8
1321794
application/pdf
TransferPlasmidsHarbouring_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/2819ff0d-75b5-4ac2-99b2-fe793eafe588/download
File
MD5
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text/plain
TransferPlasmidsHarbouring_2022.pdf.txt