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oai:repisalud.isciii.es:20.500.12105/160222025-05-08T06:44:04Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Mir-Cros, Alba author Moreno-Mingorance, Albert author Martín-Gómez, María Teresa author Abad, Raquel author Bloise, Iván author Campins, Magda author González-Praetorius, Alejandro author Gutiérrez, M Nieves author Martín-González, Héctor author Muñoz-Almagro, Carmen author Orellana, M Ángeles author de Pablos, Manuela author Roca-Grande, Josep author Rodrigo, Carlos author Rodríguez, M Elena author Uriona, Sonia author Vidal, M José author Pumarola, Tomas author Larrosa, M Nieves author González-López, Juan José author 2022-05 Bordetella pertussis not expressing pertactin has increased in countries using acellular pertussis vaccines (ACV). The deficiency is mostly caused by pertactin gene disruption by IS481. To assess the effect of the transition from whole-cell vaccine to ACV on the emergence of B. pertussis not expressing pertactin in Spain, we studied 342 isolates collected during 1986-2018. We identified 93 pertactin-deficient isolates. All were detected after introduction of ACV and represented 38% of isolates collected during the ACV period; 58.1% belonged to a genetic cluster of isolates carrying the unusual prn::del(-292, 1340) mutation. Pertactin inactivation by IS481 insertion was identified in 23.7% of pertactin-deficient isolates, arising independently multiple times and in different phylogenetic branches. Our findings support the emergence and dissemination of a cluster of B. pertussis with an infrequent mechanism of pertactin disruption in Spain, probably resulting from introduction of ACV. Emerg Infect Dis. 2022 May;28(5):967-976. 10.3201/eid2805.211958 1080-6059 Emerging infectious diseases 35447067 http://hdl.handle.net/20.500.12105/16022 Bordetella pertussis Spain Bacteria Pertussis vaccine Phylogenetic evolution Virulence factors Whooping cough Pertactin-Deficient Bordetella pertussis with Unusual Mechanism of Pertactin Disruption, Spain, 1986-2018