2026-05-22T00:46:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/160132024-10-31T11:42:31Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Köster, Pamela Carolina
author
Martínez-Nevado, Eva
author
González, Andrea
author
Abelló-Poveda, María T
author
Fernández-Bellon, Hugo
author
de la Riva-Fraga, Manuel
author
Marquet, Bertille
author
Guéry, Jean-Pascal
author
Knauf-Witzens, Tobias
author
Weigold, Annika
author
Dashti, Alejandro
author
Bailo-Barroso, Begoña
author
Imaña, Elena
author
Muadica, Aly Salimo
author
Gonzalez-Barrio, David
author
Ponce-Gordo, Francisco
author
Calero-Bernal, Rafael
author
Carmena, David
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
2023-05-08T11:58:01Z
2023-05-08T11:58:01Z
2022-01
Front Vet Sci. 2022 Jan 4;8:819887.
10.3389/fvets.2021.819887
2297-1769
Frontiers in veterinary science
35059456
http://hdl.handle.net/20.500.12105/16013
We assessed the occurrence, genetic diversity, and zoonotic potential of four protozoan (Cryptosporidium spp., Entamoeba histolytica, Entamoeba dispar, Giardia duodenalis), one stramenopile (Blastocystis sp.), one microsporidia (Enterocytozoon bieneusi), and two ciliate (Balantioides coli, Troglodytella abrassarti) intestinal parasite or commensal protist species in captive non-human primates (NHP) and their zookeepers from six European zoological gardens in France (n = 1), Germany (n = 1), and Spain (n = 4). Faecal samples from NHP (n = 454) belonging to 63 species within 35 genera and humans (n = 70) were collected at two sampling periods in each participating institution between October 2018-August 2021. Detection and species identification was accomplished by PCR and Sanger sequencing of the ssu rRNA and/or ITS genes. Sub-genotyping analyses using specific markers were conducted on isolates positive for G. duodenalis (gdh, bg, tpi) and Cryptosporidium spp. (gp60). Overall, 41.0% (186/454) and 30.0% (21/70) of the faecal samples of NHP and human origin tested positive for at least one intestinal protist species, respectively. In NHP, Blastocystis sp. was the most prevalent protist species found (20.3%), followed by G. duodenalis (18.1%), E. dispar (7.9%), B. coli and T. abrassarti (1.5% each), and Cryptosporidium spp. and E. bieneusi (0.9% each). Occurrence rates varied largely among NHP host species, sampling periods, and zoological institutions. The predominant protist species found in humans was Blastocystis sp. (25.7%), followed by Cryptosporidium spp. (2.9%), E. dispar (1.4%), and G. duodenalis (1.4%). Sequencing of PCR-positive amplicons in human and/or NHP confirmed the presence of Cryptosporidium in six isolates (C. hominis: 66.7%, C. parvum: 33.3%), G. duodenalis in 18 isolates (assemblage A: 16.7%, assemblage B: 83.3%), Blastocystis in 110 isolates (ST1:38.2%, ST2:11.8%, ST3: 18.2%, ST4: 9.1%, ST5: 17.3%, ST8: 2.7%, ST13: 0.9%), and E. bieneusi in four isolates (CM18: 75.0%, Type IV: 25.0%). Zoonotic transmission events involving Blastocystis ST1-ST4 were identified in four zoological institutions. Zoonotic transmission of C. hominis was highly suspected, but not fully demonstrated, in one of them. Monitoring of intestinal protist species might be useful for assessing health status of captive NHP and their zookeepers, and to identify transmission pathways of faecal-orally transmitted pathogens.
eng
Captive non-human primates
Commensals
Conservation
Genotyping
Intestinal parasites
Protists
Transmission
Zoonosis
Intestinal Protists in Captive Non-human Primates and Their Handlers in Six European Zoological Gardens. Molecular Evidence of Zoonotic Transmission
journal article
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
URL
https://repisalud.isciii.es/bitstreams/c4d47caf-40cd-45f7-b2f9-d94528ca0d98/download
File
MD5
8383f7496383db35278de8f955541308
971307
application/pdf
IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/e154dfed-3f12-42cc-940d-b27ff9085790/download
File
MD5
0b095c0ec0a8e76c18f5dd03bcad6ab7
189206
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Table1_IntestinalProtistsCaptiveNon-humanPrimates_2022.xlsx
URL
https://repisalud.isciii.es/bitstreams/3c0ec144-3da3-415c-adea-f8445d0b1bc6/download
File
MD5
1e2f995a41679a056decb34532691955
124879
application/pdf
Table2_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/545430d4-5273-4f8a-93b1-ebed37fe10e0/download
File
MD5
99775573220585646b3e77037643ca8f
290048
application/pdf
Table3_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/7fcec8cd-1133-4145-a5d3-9761dce58ade/download
File
MD5
6a52fb1ff0e0ebb3a816eb03f1657d48
13719
application/pdf
Table4_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/6bea0225-6b5a-496f-9186-828ed949f431/download
File
MD5
0a7e8d3a039c97e9bb2c1b570a8473b7
8704
application/pdf
Table5_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/1d97bbf4-b6c6-4cee-aa28-bfee8a5c46be/download
File
MD5
d1ff7169810d2f28fa2a2c077e3a4464
166212
application/pdf
Table6_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/6d5545e9-8ee5-4b25-a5f2-80fb5708235f/download
File
MD5
ffa184b6ec1b25797dc316ec1bc7b13a
138631
application/pdf
Table7_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/628b5dcd-2b13-4fa4-bb7f-3359b5d77add/download
File
MD5
e85a26367ae80b149adc5ca1cb2f7927
120342
application/pdf
Table8_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/510b0a78-c964-4820-8d59-63740e163da1/download
File
MD5
c9c0da51e38bffe56d94149b6045a426
96381
text/plain
IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/7fcf6ac4-2f48-4d0d-8062-1bc22d05bea5/download
File
MD5
d38380a1c551b6db33a4ff2d4286186c
2493
text/plain
Table2_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/b0b7c8ed-3913-4c5a-af4c-c0acf5b9f951/download
File
MD5
d5a9d229d6f93d045c470e1858a6d7a5
1676
text/plain
Table3_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/21065c3a-f5b3-421a-8e52-9350568f6279/download
File
MD5
28c787d53d05afdbfdf062beadee77eb
856
text/plain
Table4_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/82734b51-bdd8-4cb3-b1a9-5e3e0adc931a/download
File
MD5
5eafaeb91bfd9a8cab7e3897b91a0a9b
387
text/plain
Table5_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/cf915a72-19ee-4bd5-8fa5-dd1404c1c6af/download
File
MD5
81f7a5c6a9b1ed00bc1ca75abe56d4a8
2302
text/plain
Table6_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/1f4ec547-becc-4d8d-a423-ac1ae33479b9/download
File
MD5
6305be2f0205b27ce13989507a7e5564
2190
text/plain
Table7_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/e3b4b6d3-3bd0-4009-afe3-a501b055c0a0/download
File
MD5
37b1d8a3e81ef1b758930ba9a57651e1
1199
text/plain
Table8_IntestinalProtistsCaptiveNon-humanPrimates_2022.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/3ad78117-585d-4989-b194-9c8c103bdc3a/download
File
MD5
112c38d4e445ac6370ba4a4bf3bdbaeb
125544
text/plain
Table1_IntestinalProtistsCaptiveNon-humanPrimates_2022.xlsx.txt