2026-08-05T15:04:42Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/159752025-04-07T08:19:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Singh, Pallavi author Lonardi, Stefano author Liang, Qihua author Vydyam, Pratap author Khabirova, Eleonora author Fang, Tiffany author Gihaz, Shalev author Thekkiniath, Jose author Munshi, Muhammad author Abel, Steven author Ciampossin, Loic author Batugedara, Gayani author Gupta, Mohit author Lu, Xueqing Maggie author Lenz, Todd author Chakravarty, Sakshar author Cornillot, Emmanuel author Hu, Yangyang author Ma, Wenxiu author Gonzalez, Luis Miguel author Sanchez-Prieto, Sergio author Estrada, Karel author Sánchez-Flores, Alejandro author Montero-Clemente, Estrella author Harb, Omar S author Le Roch, Karine G author Mamoun, Choukri Ben author 2023-04-13 Babesiosis is a malaria-like disease in humans and animals that is caused by Babesia species, which are tick-transmitted apicomplexan pathogens. Babesia duncani causes severe to lethal infection in humans, but despite the risk that this parasite poses as an emerging pathogen, little is known about its biology, metabolic requirements or pathogenesis. Unlike other apicomplexan parasites that infect red blood cells, B. duncani can be continuously cultured in vitro in human erythrocytes and can infect mice resulting in fulminant babesiosis and death. We report comprehensive, detailed molecular, genomic, transcriptomic and epigenetic analyses to gain insights into the biology of B. duncani. We completed the assembly, 3D structure and annotation of its nuclear genome, and analysed its transcriptomic and epigenetics profiles during its asexual life cycle stages in human erythrocytes. We used RNA-seq data to produce an atlas of parasite metabolism during its intraerythrocytic life cycle. Characterization of the B. duncani genome, epigenome and transcriptome identified classes of candidate virulence factors, antigens for diagnosis of active infection and several attractive drug targets. Furthermore, metabolic reconstitutions from genome annotation and in vitro efficacy studies identified antifolates, pyrimethamine and WR-99210 as potent inhibitors of B. duncani to establish a pipeline of small molecules that could be developed as effective therapies for the treatment of human babesiosis. Nat Microbiol. 2023 May;8(5):845-859. 10.1038/s41564-023-01360-8 2058-5276 Nature microbiology 37055610 http://hdl.handle.net/20.500.12105/15975 Babesia duncani multi-omics identifies virulence factors and drug targets