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oai:repisalud.isciii.es:20.500.12105/159442025-04-30T10:21:44Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19613

00925njm 22002777a 4500 dc Ghosh, Prakash author Chowdhury, Rajashree author Maruf, Shomik author Picado, Albert author Hossain, Faria author Owen, Sophie I author Nath, Rupen author Baker, James author Hasnain, Md Golam author Shomik, Mohammad Sohel author Ghosh, Debashis author Rashid, Masud author Rashid, Md Utba author Sagar, Soumik Kha author Rahat, Md Abu author Basher, Ariful author Nath, Proggananda author Edwards, Thomas author Andrews, Jason R author Duthie, Malcolm S author de Souza, Dziedzom K author Adams, Emily R author Ndung'u, Joseph Mathu author Cruz, Israel author Mondal, Dinesh author 2022-10-27 Despite the availability of highly sensitive polymerase chain reaction (PCR)-based methods, the dearth of remotely deployable diagnostic tools circumvents the early and accurate detection of individuals with post-kala-azar dermal leishmaniasis (PKDL). Here, we evaluate a design-locked loop-mediated isothermal amplification (LAMP) assay to diagnose PKDL. A total of 76 snip-skin samples collected from individuals with probable PKDL (clinical presentation and a positive rK39 rapid diagnostic test (RDT)) were assessed by microscopy, qPCR, and LAMP. An equal number of age and sex-matched healthy controls were included to determine the specificity of the LAMP assay. The LAMP assay with a Qiagen DNA extraction (Q-LAMP) showed a promising sensitivity of 72.37% (95% CI: 60.91-82.01%) for identifying the PKDL cases. LAMP assay sensitivity declined when the DNA was extracted using a boil-spin method. Q-qPCR showed 68.42% (56.75-78.61%) sensitivity, comparable to LAMP and with an excellent agreement, whereas the microscopy exhibited a weak sensitivity of 39.47% (28.44-51.35%). When microscopy and/or qPCR were considered the gold standard, Q-LAMP exhibited an elevated sensitivity of 89.7% (95% CI: 78.83-96.11%) for detection of PKDL cases and Bayesian latent class modeling substantiated the excellent sensitivity of the assay. All healthy controls were found to be negative. Notwithstanding the optimum efficiency of the LAMP assay towards the detection of PKDL cases, further optimization of the boil-spin method is warranted to permit remote use of the assay. Sci Rep. 2022 Oct 27;12(1):18069. http://hdl.handle.net/20.500.12105/15944 36302782 10.1038/s41598-022-21497-6 2045-2322 Scientific reports Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis