2026-04-29T02:37:59Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/159182025-05-09T09:25:06Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc Pérez-Ginés, Víctor author Torrente-Rodríguez, Rebeca M author Montero-Calle, Ana Maria author Solis-Fernandez, Guillermo author Atance-Gómez, Pablo author Pedrero, María author Pingarrón, José M author Barderas Manchado, Rodrigo author Campuzano, Susana author 2022 The great opportunities represented by exosomes in liquid biopsy diagnostics and the relevance of CD147 protein as diagnostic and prognostic cancer biomarker led us to develop the first bio-electroanalytical platform for the determination of exosomal CD147 (exoCD147) by exploiting micro-sized magnetic beads coated with specific anti-CD147 antibodies. The captured exosomal target protein was sandwiched by specific biotin functionalized detector antibodies followed by attaching streptavidin-HRP conjugate to perform the amperometric reading using screen-printed carbon electrodes (SPCEs) as electrode transducers in the presence of hydroquinone (HQ) and H2O2. The analytical and operational characteristics achieved by implementing this simple methodology allowed the sensitive (LOD 29 pg mL-1) and selective determination of CD147 and the analysis of exoCD147 in different but inter-related real clinical scenarios including lysed and entire exosomes previously isolated from CRC cell lines with different metastatic potential. The obtained results, in agreement with those provided by ELISA and WB, proved the reliability of the developed immunosensor and its potential to isolate or identify specific subpopulations of exosomes based on the differential expression of characteristic surface biomarkers. Biosensors and Bioelectronics: X. 2022,11:100192. 2590-1370 http://hdl.handle.net/20.500.12105/15918 10.1016/j.biosx.2022.100192 Biosensors and Bioelectronics: X ExoCD147 Bio-electroanalytical platform Entire exosomes Cell lines Tackling CD147 exosome-based cell-cell signaling by electrochemical biosensing for early colorectal cancer detection