2026-04-29T19:07:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/159132025-04-22T11:45:06Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Valverde, Alejandro
author
Gordón Pidal, José M
author
Montero-Calle, Ana Maria
author
Arévalo, Beatriz
author
Serafín, Verónica
author
Calero, Miguel
author
Moreno‐Guzmán, María
author
López, Miguel Ángel
author
Escarpa, Alberto
author
Yáñez‐Sedeño, Paloma
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
author
Pingarrón, José M
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Ministerio de Educación, Cultura y Deporte (España)
funder
Instituto de Salud Carlos III
funder
Comunidad de Madrid (España)
funder
Complutense University of Madrid (España)
2023-04-27T10:11:04Z
2023-04-27T10:11:04Z
2022-02
ChemElectroChem. 2022, 9:e202200055.
10.1002/celc.202200055
2196-0216
2196-0216
ChemElectroChem
http://hdl.handle.net/20.500.12105/15913
Alzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzy matic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values<5%, and lower cost and shorter assay time (60–90 min) than other available methodologies and was applied to the analysis of plasma from healthy controls and AD patients.
eng
Alzheimer’s disease
Candidate biomarkers
Electrochemical multiplexing immunoplatform
Magnetic microbeads
Plasma
Paving the Way for Reliable Alzheimer's Disease Blood Diagnosis by Quadruple Electrochemical Immunosensing
research article
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URL
https://repisalud.isciii.es/bitstreams/ad6ac16a-9489-4f8f-a4da-12b6da4921c8/download
File
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application/pdf
PavingWayReliableAlzheimer_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/46e38827-4815-4863-ac2d-6aff604a49b7/download
File
MD5
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text/plain
PavingWayReliableAlzheimer_2022.pdf.txt