2026-07-21T15:05:40Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/159042025-06-13T10:04:36Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16970col_20.500.12105_19617
Repisalud
author
Varsaki, Athanasia
author
Ortiz, Sagrario
author
Santorum, Patricia
author
López, Pilar
author
López-Alonso, Victoria
author
Hernández, Marta
author
Abad, David
author
Rodríguez-Grande, Jorge
author
Ocampo-Sosa, Alain A
author
Martínez-Suárez, Joaquín V
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
2023-04-27T07:42:29Z
2023-04-27T07:42:29Z
2022-09-19
Animals (Basel). 2022 Sep 19;12(18):2477.
10.3390/ani12182477
2076-2615
Animals : an open access journal from MDPI
36139336
http://hdl.handle.net/20.500.12105/15904
Listeria monocytogenes is an opportunistic pathogen that is widely distributed in the environment. Here we show the prevalence and transmission of L. monocytogenes in dairy farms in the Cantabria region, on the northern coast of Spain. A total of 424 samples was collected from 14 dairy farms (5 organic and 9 conventional) and 211 L. monocytogenes isolates were recovered following conventional microbiological methods. There were no statistically significant differences in antimicrobial resistance ratios between organic and conventional farms. A clonal relationship among the isolates was assessed by pulsed field gel electrophoresis (PFGE) analysis and 64 different pulsotypes were obtained. Most isolates (89%, n = 187) were classified as PCR serogroup IVb by using a multiplex PCR assay. In this case, 45 isolates of PCR serogroup IVb were whole genome-sequenced to perform a further analysis at genomic level. In silico MLST analysis showed the presence of 12 sequence types (ST), of which ST1, ST54 and ST666 were the most common. Our data indicate that the environment of cattle farms retains a high incidence of L. monocytogenes, including subtypes involved in human listeriosis reports and outbreaks. This pathogen is shed in the feces and could easily colonize dairy products, as a result of fecal contamination. Effective herd and manure management are needed in order to prevent possible outbreaks.
eng
Listeria monocytogenes
Antimicrobial resistance
Dairy cattle
Manure
Sequence type
Virulence
Whole-genome sequencing
Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain
research article
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