2026-07-21T14:41:16Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/158822025-06-18T06:29:14Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616
Repisalud
author
Orth, Martin F
author
Surdez, Didier
author
Faehling, Tobias
author
Ehlers, Anna C
author
Marchetto, Aruna
author
Grossetête, Sandrine
author
Volckmann, Richard
author
Zwijnenburg, Danny A
author
Gerke, Julia S
author
Zaidi, Sakina
author
Alonso, Javier
author
Sastre, Ana
author
Baulande, Sylvain
author
Sill, Martin
author
Cidre-Aranaz, Florencia
author
Ohmura, Shunya
author
Kirchner, Thomas
author
Hauck, Stefanie M
author
Reischl, Eva
author
Gymrek, Melissa
author
Pfister, Stefan M
author
Strauch, Konstantin
author
Koster, Jan
author
Delattre, Olivier
author
Grünewald, Thomas G P
funder
German Cancer Aid
funder
Mehr LEBEN für krebskranke Kinder - Bettina-Bräu-Stiftung
funder
Wilhelm Sander Stiftung
funder
Friedrich Baur Stiftung
funder
Deutsche Forschungsgemeinschaft (Alemania)
funder
Federal Ministry of Education & Research (Alemania)
funder
Boehringer Ingelheim Fonds
funder
Unión Europea. Comisión Europea. H2020
funder
Agence Nationale de la Recherche (Francia)
funder
Matthias-Claudius-Stiftung
funder
Dr. Leopold und Carmen Ellinger Foundation
funder
Barbara and Wilfried Mohr Foundation
2023-04-25T06:34:29Z
2023-04-25T06:34:29Z
2022-12-06
Cell Rep. 2022 Dec 6;41(10):111761.
10.1016/j.celrep.2022.111761
2211-1247
Cell reports
36476851
http://hdl.handle.net/20.500.12105/15882
Ewing sarcoma (EwS) is characterized by EWSR1-ETS fusion transcription factors converting polymorphic GGAA microsatellites (mSats) into potent neo-enhancers. Although the paucity of additional mutations makes EwS a genuine model to study principles of cooperation between dominant fusion oncogenes and neo-enhancers, this is impeded by the limited number of well-characterized models. Here we present the Ewing Sarcoma Cell Line Atlas (ESCLA), comprising whole-genome, DNA methylation, transcriptome, proteome, and chromatin immunoprecipitation sequencing (ChIP-seq) data of 18 cell lines with inducible EWSR1-ETS knockdown. The ESCLA shows hundreds of EWSR1-ETS-targets, the nature of EWSR1-ETS-preferred GGAA mSats, and putative indirect modes of EWSR1-ETS-mediated gene regulation, converging in the duality of a specific but plastic EwS signature. We identify heterogeneously regulated EWSR1-ETS-targets as potential prognostic EwS biomarkers. Our freely available ESCLA (http://r2platform.com/escla/) is a rich resource for EwS research and highlights the power of comprehensive datasets to unravel principles of heterogeneous gene regulation by chimeric transcription factors.
eng
CP: Cancer
ChiP-seq
EWSR1-ERG
EWSR1-ETS
EWSR1-FLI1
Ewing sarcoma
Enhancer
Microsatellites
Multi-omics
Pediatric sarcoma
Tumor heterogeneity
Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation
research article
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SystematicMulti-omicsCellLine_2022.pdf
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supplementary_SystematicMulti-omicsCellLine_2022.pdf
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SystematicMulti-omicsCellLine_2022.pdf.txt
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supplementary_SystematicMulti-omicsCellLine_2022.pdf.txt