2026-07-21T14:41:57Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/158822025-06-18T06:29:14Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616

00925njm 22002777a 4500 dc Orth, Martin F author Surdez, Didier author Faehling, Tobias author Ehlers, Anna C author Marchetto, Aruna author Grossetête, Sandrine author Volckmann, Richard author Zwijnenburg, Danny A author Gerke, Julia S author Zaidi, Sakina author Alonso, Javier author Sastre, Ana author Baulande, Sylvain author Sill, Martin author Cidre-Aranaz, Florencia author Ohmura, Shunya author Kirchner, Thomas author Hauck, Stefanie M author Reischl, Eva author Gymrek, Melissa author Pfister, Stefan M author Strauch, Konstantin author Koster, Jan author Delattre, Olivier author Grünewald, Thomas G P author 2022-12-06 Ewing sarcoma (EwS) is characterized by EWSR1-ETS fusion transcription factors converting polymorphic GGAA microsatellites (mSats) into potent neo-enhancers. Although the paucity of additional mutations makes EwS a genuine model to study principles of cooperation between dominant fusion oncogenes and neo-enhancers, this is impeded by the limited number of well-characterized models. Here we present the Ewing Sarcoma Cell Line Atlas (ESCLA), comprising whole-genome, DNA methylation, transcriptome, proteome, and chromatin immunoprecipitation sequencing (ChIP-seq) data of 18 cell lines with inducible EWSR1-ETS knockdown. The ESCLA shows hundreds of EWSR1-ETS-targets, the nature of EWSR1-ETS-preferred GGAA mSats, and putative indirect modes of EWSR1-ETS-mediated gene regulation, converging in the duality of a specific but plastic EwS signature. We identify heterogeneously regulated EWSR1-ETS-targets as potential prognostic EwS biomarkers. Our freely available ESCLA (http://r2platform.com/escla/) is a rich resource for EwS research and highlights the power of comprehensive datasets to unravel principles of heterogeneous gene regulation by chimeric transcription factors. Cell Rep. 2022 Dec 6;41(10):111761. 10.1016/j.celrep.2022.111761 2211-1247 Cell reports 36476851 http://hdl.handle.net/20.500.12105/15882 CP: Cancer ChiP-seq EWSR1-ERG EWSR1-ETS EWSR1-FLI1 Ewing sarcoma Enhancer Microsatellites Multi-omics Pediatric sarcoma Tumor heterogeneity Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation