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oai:repisalud.isciii.es:20.500.12105/157842024-09-27T09:48:07Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Dalmasso, Giovanni author Musy, Marco author Niksic, Martina author Robert-Moreno, Alexandre author Badia-Careaga, Claudio author Sanz-Ezquerro, Juan Jose author Sharpe, James author 2022-09-12 Normal organogenesis cannot be recapitulated in vitro for mammalian organs, unlike in species including Drosophila and zebrafish. Available 3D data in the form of ex vivo images only provide discrete snapshots of the development of an organ morphology. Here, we propose a computer-based approach to recreate its continuous evolution in time and space from a set of 3D volumetric images. Our method is based on the remapping of shape data into the space of the coefficients of a spherical harmonics expansion where a smooth interpolation over time is simpler. We tested our approach on mouse limb buds and embryonic hearts. A key advantage of this method is that the resulting 4D trajectory can take advantage of all the available data while also being able to interpolate well through time intervals for which there are little or no data. This allows for a quantitative, data-driven 4D description of mouse limb morphogenesis. Dev Cell. 2022 Sep 12;57(17):2140-2150.e5 10.1016/j.devcel.2022.08.005 1878-1551 Developmental cell 36055247 http://hdl.handle.net/20.500.12105/15784 4D reconstruction of murine developmental trajectories using spherical harmonics.