2026-04-29T12:23:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/157802024-09-27T08:50:13Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Ahmed, Suhail
author
Kurusamy, Sathishkumar
author
David, Ezra Leander Santhosh
author
Khan, Kinza
author
Kalyanakrishnan, Krithika
author
Ian-Gobo, Miebaka
author
Kola, Teja Manidhar
author
Wilkinson, Robert N
author
Kannappan, Vinodh
author
Wang, Weiguang
author
Gómez, Manuel J
author
Redondo, Juan Miguel
author
Cotton, James
author
Armesilla, Angel L
funder
Fundación La Caixa
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Fundación ProCNIC
2023-04-12T09:30:50Z
2023-04-12T09:30:50Z
2022-08-30
Sci Rep. 2022 Aug 30;12(1):14730
10.1038/s41598-022-19172-x
2045-2322
Scientific reports
36042288
http://hdl.handle.net/20.500.12105/15780
Angiogenesis is a multi-factorial physiological process deregulated in human diseases characterised by excessive or insufficient blood vessel formation. Emerging evidence highlights a novel role for microRNAs as regulators of angiogenesis. Previous studies addressing the effect of miR-133a expression in endothelial cells during blood vessel formation have reported conflicting results. Here, we have assessed the specific effect of mature miR-133a strands in angiogenesis and the expression of endothelial angiogenic genes. Transfection of miR-133a-3p or -5p mimics in primary human endothelial cells significantly inhibited proliferation, migration, and tubular morphogenesis of transfected cells. Screening of gene arrays related to angiogenic processes, and further validation by TaqMan qPCR, revealed that aberrant expression of miR-133a-3p led to a decrease in the expression of genes encoding pro-angiogenic molecules, whilst increasing those with anti-angiogenic functions. Ingenuity Pathway Analysis of a collection of genes differentially expressed in cells harbouring miR-133a-3p, predicted decreased cellular functions related to vasculature branching and cell cycle progression, underlining the inhibitory role of miR-133a-3p in angiogenic cellular processes. Our results suggest that controlled delivery of miR-133a-3p mimics, or antagomirs in diseased endothelial cells, might open new therapeutic interventions to treat patients suffering from cardiovascular pathologies that occur with excessive or insufficient angiogenesis.
eng
Aberrant expression of miR-133a in endothelial cells inhibits angiogenesis by reducing pro-angiogenic but increasing anti-angiogenic gene expression.
journal article
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Aberrant expression of miR_133 Sci Rep 2022.pdf
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https://repisalud.isciii.es/bitstreams/db979a24-5071-4cb4-8a16-7263238dda77/download
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Aberrant expression of miR_133 Sci Rep 2022.pdf.txt