2026-04-29T22:51:01Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/157642024-09-27T08:26:47Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Alvarez, Maria Soledad
author
Nunez, Estefania
author
Fuertes-Agudo, Marina
author
Cucarella, Carme
author
Fernandez-Velasco, Maria
author
Boscá, Lisardo
author
Vazquez, Jesus
author
Rossignol, Rodrigue
author
Martin-Sanz, Paloma
author
Casado, Marta
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Generalitat Valenciana (España)
funder
Centro de Investigación Biomédica en Red - CIBERCV (Enfermedades Cardiovasculares)
funder
Consejo Superior de Investigaciones Científicas (España)
2023-04-11T14:31:23Z
2023-04-11T14:31:23Z
2022-11-03
Int J Mol Sci. 2022 Nov 3;23(21):13476
10.3390/ijms232113476
1422-0067
International journal of molecular sciences
36362254
http://hdl.handle.net/20.500.12105/15764
The biochemical mechanisms of cell injury and myocardial cell death after myocardial infarction remain unresolved. Cyclooxygenase 2 (COX-2), a key enzyme in prostanoid synthesis, is expressed in human ischemic myocardium and dilated cardiomyopathy, but it is absent in healthy hearts. To assess the role of COX-2 in cardiovascular physiopathology, we developed transgenic mice that constitutively express functional human COX-2 in cardiomyocytes under the control of the α-myosin heavy chain promoter. These animals had no apparent phenotype but were protected against ischemia-reperfusion injury in isolated hearts, with enhanced functional recovery and diminished cellular necrosis. To further explore the phenotype of this animal model, we carried out a differential proteome analysis of wild-type vs. transgenic cardiomyocytes. The results revealed a tissue-specific proteomic profile dominated by mitochondrial proteins. In particular, an increased expression of respiratory chain complex IV proteins was observed. This correlated with increased catalytic activity, enhanced respiratory capacity, and increased ATP levels in the heart of COX-2 transgenic mice. These data suggest a new link between COX-2 and mitochondria, which might contribute to the protective cardiac effects of COX-2 against ischemia-reperfusion injury.
eng
Quantitative Proteomics Analysis Reveals That Cyclooxygenase-2 Modulates Mitochondrial Respiratory Chain Complex IV in Cardiomyocytes.
journal article
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Quantitative Proteomics Analysis Reveals Int J Mol Sci 2022.pdf
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Quantitative Proteomics Analysis Reveals Int J Mol Sci 2022.pdf.txt