2026-04-29T16:56:10Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/157402024-09-21T10:55:53Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Céspedes, Pablo F author Jainarayanan, Ashwin author Fernández-Messina, Lola author Valvo, Salvatore author Saliba, David G author Kurz, Elke author Kvalvaag, Audun author Chen, Lina author Ganskow, Charity author Colin-York, Huw author Fritzsche, Marco author Peng, Yanchun author Dong, Tao author Johnson, Errin author Siller-Farfán, Jesús A author Dushek, Omer author Sezgin, Erdinc author Peacock, Ben author Law, Alice author Aubert, Dimitri author Engledow, Simon author Attar, Moustafa author Hester, Svenja author Fischer, Roman author Sánchez-Madrid, Francisco author Dustin, Michael L author 2022-06-16 The immunological synapse is a molecular hub that facilitates the delivery of three activation signals, namely antigen, costimulation/corepression and cytokines, from antigen-presenting cells (APC) to T cells. T cells release a fourth class of signaling entities, trans-synaptic vesicles (tSV), to mediate bidirectional communication. Here we present bead-supported lipid bilayers (BSLB) as versatile synthetic APCs to capture, characterize and advance the understanding of tSV biogenesis. Specifically, the integration of juxtacrine signals, such as CD40 and antigen, results in the adaptive tailoring and release of tSV, which differ in size, yields and immune receptor cargo compared with steadily released extracellular vesicles (EVs). Focusing on CD40L+ tSV as model effectors, we show that PD-L1 trans-presentation together with TSG101, ADAM10 and CD81 are key in determining CD40L vesicular release. Lastly, we find greater RNA-binding protein and microRNA content in tSV compared with EVs, supporting the specialized role of tSV as intercellular messengers. Nat Commun. 2022 Jun 16;13(1):3460 10.1038/s41467-022-31160-3 2041-1723 Nature communications 35710644 http://hdl.handle.net/20.500.12105/15740 T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles.