2026-04-29T00:59:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/157352024-09-27T07:56:27Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
de Pedro, María Ángeles
author
Pulido, María
author
Marinaro, Federica
author
Álvarez, Verónica
author
Báez-Díaz, Claudia
author
Blanco, Virginia
author
Silla-Castro, Juan Carlos
author
Sanchez-Cabo, Fatima
author
Sánchez-Margallo, Francisco Miguel
author
Crisóstomo, Verónica
author
Casado, Javier G
author
López, Esther
funder
Instituto de Salud Carlos III
funder
Banco Santander
funder
Unión Europea. Fondo Social Europeo (ESF/FSE)
funder
Government of Extremadura (España)
2023-04-03T12:02:47Z
2023-04-03T12:02:47Z
2022-05-11
Biomedicines. 2022 May 11;10(5):1117
2227-9059
http://hdl.handle.net/20.500.12105/15735
35625854
10.3390/biomedicines10051117
Biomedicines
Acute myocardial infarction (AMI) is a manifestation of ischemic heart disease where the immune system plays an important role in the re-establishment of homeostasis. We hypothesize that the anti-inflammatory activity of secretomes from menstrual blood-derived mesenchymal stromal cells (S-MenSCs) and IFNγ/TNFα-primed MenSCs (S-MenSCs*) may be considered a therapeutic option for the treatment of AMI. To assess this hypothesis, we have evaluated the effect of S-MenSCs and S-MenSCs* on cardiac function parameters and the involvement of immune-related genes using a porcine model of AMI. Twelve pigs were randomly divided into three biogroups: AMI/Placebo, AMI/S-MenSCs, and AMI/S-MenSCs*. AMI models were generated using a closed chest coronary occlusion-reperfusion procedure and, after 72 h, the different treatments were intrapericardially administered. Cardiac function parameters were monitored by magnetic resonance imaging before and 7 days post-therapy. Transcriptomic analyses in the infarcted tissue identified 571 transcripts associated with the Gene Ontology term Immune response, of which 57 were differentially expressed when different biogroups were compared. Moreover, a prediction of the interactions between differentially expressed genes (DEGs) and miRNAs from secretomes revealed that some DEGs in the infarction area, such as STAT3, IGFR1, or BCL6 could be targeted by previously identified miRNAs in secretomes from MenSCs. In conclusion, the intrapericardial administration of secretome early after infarction has a significant impact on the expression of immune-related genes in the infarcted myocardium. This confirms the immunomodulatory potential of intrapericardially delivered secretomes and opens new therapeutic perspectives in myocardial infarction treatment.
eng
Intrapericardial Administration of Secretomes from Menstrual Blood-Derived Mesenchymal Stromal Cells: Effects on Immune-Related Genes in a Porcine Model of Myocardial Infarction.
journal article
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URL
https://repisalud.isciii.es/bitstreams/c6cc7d27-b276-4a9e-9383-36c638797736/download
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Intrapericardial Administration of Secretomes Biomedicines 2022.pdf
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https://repisalud.isciii.es/bitstreams/5189026b-03b4-450f-a111-8050c2e32827/download
File
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Intrapericardial Administration of Secretomes Biomedicines 2022.pdf.txt