2026-07-21T07:23:04Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/157342024-09-21T09:40:02Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Chen, Ao author Liao, Sha author Cheng, Mengnan author Ma, Kailong author Wu, Liang author Lai, Yiwei author Qiu, Xiaojie author Yang, Jin author Xu, Jiangshan author Hao, Shijie author Wang, Xin author Lu, Huifang author Chen, Xi author Liu, Xing author Huang, Xin author Li, Zhao author Hong, Yan author Jiang, Yujia author Peng, Jian author Liu, Shuai author Shen, Mengzhe author Liu, Chuanyu author Li, Quanshui author Yuan, Yue author Wei, Xiaoyu author Zheng, Huiwen author Feng, Weimin author Wang, Zhifeng author Liu, Yang author Wang, Zhaohui author Yang, Yunzhi author Xiang, Haitao author Han, Lei author Qin, Baoming author Guo, Pengcheng author Lai, Guangyao author Muñoz-Cánoves, Pura author Maxwell, Patrick H author Thiery, Jean Paul author Wu, Qing-Feng author Zhao, Fuxiang author Chen, Bichao author Li, Mei author Dai, Xi author Wang, Shuai author Kuang, Haoyan author Hui, Junhou author Wang, Liqun author Fei, Ji-Feng author Wang, Ou author Wei, Xiaofeng author Lu, Haorong author Wang, Bo author Liu, Shiping author Gu, Ying author Ni, Ming author Zhang, Wenwei author Mu, Feng author Yin, Ye author Yang, Huanming author Lisby, Michael author Cornall, Richard J author Mulder, Jan author Uhlén, Mathias author Esteban, Miguel A author Li, Yuxiang author Liu, Longqi author Xu, Xun author Wang, Jian author 2022-05-12 Spatially resolved transcriptomic technologies are promising tools to study complex biological processes such as mammalian embryogenesis. However, the imbalance between resolution, gene capture, and field of view of current methodologies precludes their systematic application to analyze relatively large and three-dimensional mid- and late-gestation embryos. Here, we combined DNA nanoball (DNB)-patterned arrays and in situ RNA capture to create spatial enhanced resolution omics-sequencing (Stereo-seq). We applied Stereo-seq to generate the mouse organogenesis spatiotemporal transcriptomic atlas (MOSTA), which maps with single-cell resolution and high sensitivity the kinetics and directionality of transcriptional variation during mouse organogenesis. We used this information to gain insight into the molecular basis of spatial cell heterogeneity and cell fate specification in developing tissues such as the dorsal midbrain. Our panoramic atlas will facilitate in-depth investigation of longstanding questions concerning normal and abnormal mammalian development. Cell. 2022 May 12;185(10):1777-1792.e21 10.1016/j.cell.2022.04.003 1097-4172 Cell 35512705 http://hdl.handle.net/20.500.12105/15734 Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays.