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oai:repisalud.isciii.es:20.500.12105/157322024-09-27T08:15:11Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Sevilla-Movilla, Silvia author Fuentes, Patricia author Rodríguez-García, Yaiza author Arellano-Sánchez, Nohemi author Krenn, Peter W author de Val, Soledad Isern author Montero-Herradón, Sara author García-Ceca, Javier author Burdiel-Herencia, Valeria author Gardeta, Sofía R author Aguilera-Montilla, Noemí author Barrio-Alonso, Celia author Crainiciuc, Georgiana author Bouvard, Daniel author García-Pardo, Angeles author Zapata, Agustin G author Hidalgo, Andres author Fässler, Reinhard author Carrasco, Yolanda R author Toribio, Maria L author Teixidó, Joaquin author 2022-08 ICAP-1 regulates β1-integrin activation and cell adhesion. Here, we used ICAP-1-null mice to study ICAP-1 potential involvement during immune cell development and function. Integrin α4β1-dependent adhesion was comparable between ICAP-1-null and control thymocytes, but lack of ICAP-1 caused a defective single-positive (SP) CD8+ cell generation, thus, unveiling an ICAP-1 involvement in SP thymocyte development. ICAP-1 bears a nuclear localization signal and we found it displayed a strong nuclear distribution in thymocytes. Interestingly, there was a direct correlation between the lack of ICAP-1 and reduced levels in SP CD8+ thymocytes of Runx3, a transcription factor required for CD8+ thymocyte generation. In the spleen, ICAP-1 was found evenly distributed between cytoplasm and nuclear fractions, and ICAP-1-/- spleen T and B cells displayed upregulation of α4β1-mediated adhesion, indicating that ICAP-1 negatively controls their attachment. Furthermore, CD3+ - and CD19+ -selected spleen cells from ICAP-1-null mice showed reduced proliferation in response to T- and B-cell stimuli, respectively. Finally, loss of ICAP-1 caused a remarkable decrease in marginal zone B- cell frequencies and a moderate increase in follicular B cells. Together, these data unravel an ICAP-1 involvement in the generation of SP CD8+ thymocytes and in the control of marginal zone B-cell numbers. Eur J Immunol. 2022 Aug;52(8):1228-1242 http://hdl.handle.net/20.500.12105/15732 35491946 10.1002/eji.202149560 1521-4141 European journal of immunology ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice.