2026-04-19T15:27:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/157132024-09-27T09:57:03Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Mashinchian, Omid
author
Hong, Xiaotong
author
Michaud, Joris
author
Migliavacca, Eugenia
author
Lefebvre, Gregory
author
Boss, Christophe
author
De Franceschi, Filippo
author
Le Moal, Emmeran
author
Collerette-Tremblay, Jasmin
author
Isern, Joan
author
Metairon, Sylviane
author
Raymond, Frederic
author
Descombes, Patrick
author
Bouche, Nicolas
author
Muñoz-Cánoves, Pura
author
Feige, Jerome N
author
Bentzinger, C Florian
funder
Canadian Institutes of Health Research
funder
National Science and Research Council of Canada
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Fundación La Caixa
funder
Unión Europea. Comisión Europea. H2020
funder
Association française pour la myopathie
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2023-03-27T10:50:03Z
2023-03-27T10:50:03Z
2022-03-04
Elife. 2022 Mar 4;11:e57393
http://hdl.handle.net/20.500.12105/15713
35245177
10.7554/eLife.57393
2050-084X
eLife
Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here, we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging in vivo independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence, and suppress myogenic differentiation. Importantly, in vitro profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus, in vivo transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy.
eng
In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging.
journal article
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https://repisalud.isciii.es/bitstreams/c1ea31d6-8bb9-44c1-b538-41b8e1be36b2/download
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In vivo transcriptomic profiling Elife 2022.pdf
URL
https://repisalud.isciii.es/bitstreams/fee00368-c16f-4019-a752-05d42f8b5d75/download
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In vivo transcriptomic profiling Elife 2022.pdf.txt